基于磁性花粉状纳米硅球的循环肿瘤细胞高效捕获  

Magnetic pollen-like silica nanoparticles for efficient capture of circulating tumor cells

作　　者：靳荣荣 高明霞[1] 张祥民[1] Rongrong Jin;Mingxia Gao;Xiangmin Zhang(Department of Chemistry,Fudan University,Shanghai 200433,China)

机构地区：[1]复旦大学化学系,上海200433

出　　处：《中国科学：化学》2022年第3期515-522,共8页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(编号:21974023,21475027);国家重点研发计划(编号:2016YFA0501401,2016YFA0501402,2017YFA0505003)资助项目。

摘　　要：本文设计合成了一种适配体修饰的磁性花粉状纳米硅球用于循环肿瘤细胞(CTCs)的高效捕获.此纳米硅球表面特有的“硅毛刺”结构不仅可为后续适配体的修饰提供更多的结合位点,而且可以与细胞表面的微绒毛及伪足相互作用,促进细胞的铺展和伪足的延伸,可进一步提高CTCs的捕获效率.实验结果表明,此纳米硅球的捕获效率高达85%,且捕获后细胞的存活率接近100%.将此纳米材料用于模拟实际样中CTCs的检测分析,其捕获效率为53%~83%,研究结果表明,即使在体系复杂的样品中,该纳米材料仍具有良好的捕获性能,为CTCs的分离纯化提供了新的方法与技术.Aptamer-modified magnetic pollen-like silica nanoparticles were synthesized and used as a substrate for the efficient capture of circulating tumor cells. Silica spikes on the surface of nanoparticles can not only provide more binding sites for the modification of aptamer, but also interact with the microvillus and pseudopods on the cell surface,thus improving the capture efficiency of circulating tumor cells(CTCs). The capture efficiency was up to 85%, and the cell viability was nearly 100%. The mimic clinical sample array showed that this strategy achieved a good capture performance even in the complex system and showed a good potential in the clinical applications.

关 键 词：磁性花粉状纳米硅球 循环肿瘤细胞 高效捕获 

分 类 号：R730[医药卫生—肿瘤] TB383.1[医药卫生—临床医学]