siRNA抑制牛睾丸细胞Nrf2基因表达的研究  

Inhibition of Nrf2 Gene Expression in Primary Bovine Testicular Cells by siRNA

作　　者：胡修忠 向敏 余婕 刘辰晖 夏瑜 陶弼菲 周源 王定发 程蕾 HU Xiu-zhong;XIANG Min;YU Jie;LIU Chen-hui;XIA Yu;TAO Bi-fei;ZHOU Yuan;WANG Ding-fa;CHENG Lei(Insitute of Animal Husbandry and Veterinary,Wuhan Academy of Agricultural Sciences,Wuhan 430208)

机构地区：[1]武汉市农业科学院畜牧兽医科学研究所,武汉430208

出　　处：《中国奶牛》2022年第3期11-15,共5页China Dairy Cattle

基　　金：湖北省自然科学基金项目(2019CFC872)。

摘　　要：为了探讨Nrf2基因被干扰后对牛睾丸细胞的影响,试验采用了实时荧光定量PCR和Western boltting方法检测了牛睾丸细胞中HO-1和NQO1基因的表达。结果显示,设计的siRNA-1672在终浓度为50nmol/L时抑制效果较好,Nrf2基因表达显著下降,HO-1和NQO1基因在mRNA水平上表达显著下降。Nrf2被干扰72h后,HO-1和NQO1蛋白质水平表达水平明显下降。说明Nrf2对牛睾丸细胞中HO-1和NQO1基因的表达存在调控作用。In order to investigate the effect of Nrf2 gene interference on primary bovine testicular cells, the expression of HO-1 and NQO1 in primary bovine testicular cells were detected by real-time quantitative PCR and Western bolting. The results showed that the designed siRNA-1672 had a better inhibitory effect at the final concentration of 50 nmol/L, the expression of Nrf2 gene decreased significantly, and the expression of HO-1 and NQO1 genes was significantly decreased at the mRNA level. After 72 hours of interference, the expression levels of HO-1 and NQO1 protein decreased significantly. This indicates that Nrf2 regulates the expression of HO-1 and NQO1 genes in primary bovine testicular cells.

关 键 词：牛睾丸细胞 RNA干扰 NRF2 HO-1 NQO1 

分 类 号：S823.3[农业科学—畜牧学]