浙江省新生儿脐带血血常规相关指标和各型血红蛋白质量百分比参考值的确定及其对血红蛋白病诊断的意义分析  

Reference Values of Umbilical Cord Blood Routine and Hemoglobin Capillary Electrophoresis in Newborns in Zhejiang Province and Their Significance in the Diagnosis of Hemoglobinopathy

作　　者：吴飞 沈九龙 邓刚 WU Fei;SHEN Jiu-long;DENG Gang(Zhejiang Cord Blood Bank,311100)

机构地区：[1]浙江省脐带血造血干细胞库,311100

出　　处：《临床输血与检验》2022年第2期239-243,共5页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的通过大规模检测浙江省足月新生儿脐带血血常规相关指标和各型血红蛋白的质量百分比(HbA%、HbF%、HbA_(2)%),确定血常规相关指标和各型血红蛋白的参考值范围,为新生儿血红蛋白病及其他疾病的筛查提供参考。方法用血细胞分析仪和血红蛋白毛细管电泳检测合法采集的足月健康新生儿捐献脐血1788份。应用百分位数法界定WBC、RBC、Hb浓度、MCV、MCH、MCHC、HbA%、HbF%、HbA_(2)%的双侧95%可信区间的正常参考值范围,用t检验或者方差分析判断孕周和Hb浓度对HbA%、HbF%、HbA_(2)%的影响。结果浙江省足月健康新生儿脐带血WBC、RBC、Hb浓度、MCV、MCH,MCHC的参考值范围分别为:(9.7~23.4)×10^(9)/L、(3.5~5.0)×10^(12)/L、124~178 g/L、111~131 fl、33~39 pg、280~315 g/L;血红蛋白HbA%的参考值范围为11.1%~32.1%、HbF%的参考值范围为67.5%~88.9%;HbA_(2)%的参考值范围为0~0.4%;孕周和Hb浓度对HbA%、HbF%、HbA_(2)%有影响,新生儿MCV、MCH、产妇年龄、新生儿性别与体重对HbA%、HbF%、HbA_(2)%无明显影响。结论浙江省新生儿脐带血血常规相关指标及各型血红蛋白质量百分比的参考值范围可以为疾病的诊断提供依据;孕周的长短和Hb浓度会影响新生儿HbA、HbF、HbA_(2)的质量百分比。Objective We investigated the relevant indexes of routine umbilical cord blood and the mass percentage of various types of hemoglobin(HbA%,HbF%,and HbA_(2)%)of full~term newborns in Zhejiang Province to determine their reference value ranges,and therefore provide a reference for the screening of neonatal hemoglobinopathy and other diseases.Methods Blood routine and hemoglobin capillary electrophoresis were used to detect 1788 cord blood donated by full~term healthy newborns.The percentile method was used to define the normal reference value ranges of bilateral 95% confidence intervals of WBC,RBC,Hb concentration,MCV,MCH,MCHC,HbA%,HbF and HbA_(2)%,and T~test or analysis of variance was used to judge the effects of gestational weeks and Hb concentration on HbA%,HbF% and HbA_(2)%.Results The reference values of WBC,RBC,Hb,MCV,MCH and MCHC in cord blood of full~term healthy newborns in Zhejiang Province were as follows:(9.7~23.4)×10^(9)pieces/L,(3.5~5.0)×10^(12)PCS/L,124~178 g/L,111~131 fl,33~39 pg/cell,and 280~315 g/L.The reference value range was 11.1%~32.1%in hemoglobin HbA%,67.5%~88.9%in HbF%,and 0~0.4%in HbA_(2)%.Gestational age and Hb concentration had effects on HbA%,HbF% and HbA_(2)%,but neonatal MCV,MCH,maternal age,neonatal gender and weight not.Conclusion The reference value ranges of related indexes of routine umbilical cord blood and hemoglobin capillary electrophoresis in newborns in Zhejiang Province can provide a basis for the diagnosis of diseases.Gestational weeks and Hb concentration may affect the mass percentage of HbA%,HbF%and HbA_(2)%of newborns.

关 键 词：脐带血 血常规 毛细管电泳 血红蛋白病 

分 类 号：R722.1[医药卫生—儿科]