甲基转移样酶1在胶质母细胞瘤中高表达并与促胶质母细胞瘤生长有关  

High expression of methyltransferase-like 1 in glioblastoma and is related to its role in promoting glioblastoma growth

作　　者：张旭[1] 曾智[2] 熊晓星[1] Zhang Xu;Zeng Zhi;Xiong Xiaoxing(Department of Neurosurgery,Renmin Hospital of Wuhan University,Wuhan 430060,China;Department of Pathology,Renmin Hospital of Wuhan University,Wuhan 430060,China)

机构地区：[1]武汉大学人民医院神经外科,武汉430060 [2]武汉大学人民医院病理科,武汉430060

出　　处：《中国组织化学与细胞化学杂志》2021年第6期507-514,共8页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金项目(81870939)。

摘　　要：目的探讨甲基转移样酶1(methyltransferase-like 1,METTL1)在人胶质母细胞瘤中的表达及其临床意义。方法通过免疫荧光双标方法观察正常人脑组织中METTL1蛋白的表达定位。采用TCGA数据库分析比较METTL1 mRNA在胶质母细胞瘤及正常人脑组织中的表达差异。选取60例人胶质母细胞瘤及10例正常人脑组织,采用组织芯片和免疫组织化学染色检测METTL1表达,并分析该蛋白表达与患者临床病理特征的关系。结果免疫荧光染色显示正常脑组织中METTL1蛋白主要富集于细胞核,且在GFAP标记的胶质细胞胞核内的表达强度显著弱于NeuN标记的神经元细胞。TCGA数据显示,METTL1 mRNA在胶质母细胞瘤中的表达明显高于正常人脑组织。免疫组织化学显示METTL1蛋白在胶质母细胞瘤组织中的阳性表达率显著高于正常人脑组织中的胶质细胞。METTL1表达与胶质母细胞瘤患者年龄、性别、发病半球及病灶原/复发性质等临床病理特征无显著相关性。生物信息学分析显示METTL1 mRNA表达与患者总体生存时间无关,降低METTL1在胶质瘤细胞系内表达可抑制肿瘤细胞生长水平。结论METTL1促进胶质母细胞瘤细胞生长,其高水平表达与胶质母细胞瘤的发生相关,但其在生存预后的预测方面作用有限。Objective To investigate the expression of methyltransferase-like 1(METTL1)in human glioblastoma and its clinical significance.Methods Immunofluorescence staining was used to observe the expression of METTL1 protein in normal human brain tissues.The expression of METTL1 mRNA in glioblastoma and normal human brain tissues was analyzed by TCGA database.The expression of METTL1 protein in 54 human glioblastoma and 10 normal human brain tissues was detected by tissue microarray and immunohistochemical staining,and the relationship between the expression of METTL1 protein and the clinicopathological characteristics was analyzed.Results Immunofluorescence staining showed that METTL1 protein was concentrated located in the nucleus of GFAP labeled glial cells,and the expression of METTL1 protein was weaker than that of NeuN labeled neurons.TCGA data showed that the expression of METTL1 mRNA in glioblastoma was significantly higher than that in normal human brain tissue.Immunohistochemistry showed that the positive expression rate of METTL1 was 50%significantly higher than that of glial cells in normal human brain.The expression of METTL1 protein was not correlated with the clinicopathological features of glioblastoma patients,such as age,gender,hemisphere and the nature of the lesion origin/recurrence.Bioinformatics analysis showed that the expression of METTL1 mRNA was not related to overall survival time of glioblastoma patients.Decreasing the expression of METTL1 inhibited the growth of glioblastoma cells.Conclusion METTL1 promotes the growth of glioblastoma cells,which is associated with glioblastoma genesis,but its prognostic role in survival is limited.

关 键 词：胶质母细胞瘤 甲基化 甲基转移样酶1 生物信息学 

分 类 号：R365[医药卫生—病理学]