猪链球菌2型分离鉴定及其对小鼠的致病性分析  

Isolation and Identification of Streptococcus suis Type 2 and Pathogenicity to Mice

作　　者：刘娜 于江[1,2] 李建达[2] 张玉玉[2] 杨灵芝 任素芳[2] 丛雁芳 郭立辉[2] 陈智[2] 吕婷婷 安利国[1] 吴家强[1,2] Liu Na;Yu Jiang;Li Jianda;Zhang Yuyu;Yang Lingzhi;Ren Sufang;Cong Yanfang;Guo Lihui;Chen Zhi;LüTingting;An Liguo;Wu Jiaqiang(College of Life Sciences,Shandong Normal University,Jinan250014,China;Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences/Shandong Key Laboratory of Animal Disease Control and Breeding,Jinan250100,China;Shandong Binzhou Wohua Bioengineering Co.,Ltd.,Binzhou256606,China;Qingdao Vland Biotech Co.,Ltd.,Qingdao266114,China)

机构地区：[1]山东师范大学生命科学学院,山东济南250014 [2]山东省农业科学院畜牧兽医研究所/山东省畜禽疫病防治与繁育重点实验室,山东济南250100 [3]山东滨州沃华生物工程有限公司,山东滨州256606 [4]青岛蔚蓝生物股份有限公司,山东青岛266114

出　　处：《山东农业科学》2022年第3期137-142,共6页Shandong Agricultural Sciences

基　　金：国家自然科学基金项目(32070178);山东省重点研发计划项目(2020CXGC010801);山东省生猪产业技术体系项目(SDAIT-08-01);山东省农业重大应用技术创新项目(SD2019XM007);国家万人计划项目(W03020496);山东省农业科学院农业科技创新工程项目(CXGC2021C04,CXGC2021A12);山东省自然科学基金面上项目(ZR2020MC177);山东省重点研发计划(农业良种工程)项目。

摘　　要：为了解猪链球菌2型菌株对昆明小鼠的致病性,并建立其感染昆明小鼠模型,本试验分别取2头疑似患链球菌病的病死猪脑组织、血液分别涂布于含5%胎牛血清的TSA固体培养基中进行细菌分离;并对分离获得的细菌进行形态观察、PCR鉴定、药物敏感性试验、小鼠致病性试验及组织病理切片观察。结果显示,分离菌为革兰氏阳性短链球菌,经PCR鉴定为猪链球菌2型,命名为LY株;药敏试验证实LY株对青霉素、红霉素、氯霉素、苯唑西林、环丙沙星、诺氟沙星敏感,对四环素、多粘菌素B、克林霉素不敏感;LY株对小鼠致死剂量为2.2×10^(9) cfu/只;组织病理切片观察发现小鼠肺脏、脾脏及心肌均出现不同程度的病变。本研究明确了山东省猪链球2型毒株的毒力,为疫苗候选菌株的筛选提供了理论依据。In order to understand the pathogenicity of Streptococcus suis type 2 strain to Kunming mice and establish its infection model,the brain tissue and blood of two dead pigs suspected of S.suis were coated in TSA solid medium containing 5%fetal bovine serum for bacterial isolation;the isolated bacterum was identified by morphology observation,PCR identification,drug sensitivity test,mouse pathogenicity test and histopathological section observation.The results showed that the isolated bacterum belonged to Gram-positive Streptococcus,which was identified as S.suis type 2 by PCR and named LY strain.The drug sensitivity test showed the LY strain was sensitive to penicillin,erythromycin,chloramphenicol,oxacillin,ciprofloxacin and norfloxacin,but resistant to tetracycline,polymyxin B and clindamycin;the lethal dose to one mice was 2.2×10^(9) cfu.The tissue section observation found that there were different degrees of lesions in lung,spleen and myocardium.This study clarified the virulence of S.suis type 2 in Shandong Province,and provided a theoretical basis for screening of vaccine candidate strains.

关 键 词：猪链球菌2型 分离鉴定 小鼠 致病性 

分 类 号：S852.611[农业科学—基础兽医学]