纤维素微球色谱介质制备、孔结构调控及聚合物改性  

Preparation, pore structure control, and polymer modification of cellulose microsphere chromatography media

作　　者：王珊珊 廖雨昕 乔亮智 杜开峰[1] WANG Shanshan;LIAO Yuxin;QIAO Liangzhi;DU Kaifeng(School of Chemical Engineering,Sichuan University,Chengdu 610065,China)

机构地区：[1]四川大学化学工程学院,四川成都610065

出　　处：《生物加工过程》2022年第2期217-225,共9页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金(21676170)。

摘　　要：作为色谱技术的核心,高效色谱介质的研制与开发是提高生物大分子纯化效率的重要手段之一。纤维素微球是最早应用于生物分离的色谱介质之一。这主要是因为纤维素的基本结构为多糖化合物,表面含有大量羟基,易于修饰功能配基,且非特异性吸附弱,特别适合不稳定生物大分子的分离纯化。本文综述了纤维素微球色谱介质的研究新进展。首先简要介绍了纤维素微球的制备方法,比较了不同方法在制备微球尺寸粒径等方面的区别;然后重点介绍了纤维素微球色谱高能化的两种策略,包括孔结构调控和聚合物配基改性以及作用机制;最后,在当前研究现状的基础上对高性能纤维素微球色谱介质的制备做出了合理展望。Chromatographic media are considered as the core of chromatography and developing high-performance chromatographic media is one of the important means to improve chromatographic performances.Cellulose microspheres, as one of the common chromatography media, are widely used in bioseparation field because of their abundant hydroxyl groups for easy modification and weak non-specific adsorption.In this review, we briefly introduce the preparation strategies of cellulose microspheres and discuss the morphology difference of the corresponding cellulose microsphere.In addition, we address two strategies for high-performance cellulose microsphere as chromatography media, including pore structure control and polymer modification.Finally, on the basis of the current researches, we indicate prospect for the preparation of high-performance cellulose microspheres in the chromatography field.

关 键 词：纤维素微球 色谱介质 吸附 生物分离 

分 类 号：O63[理学—高分子化学]