蛋白酶抑制剂BMS-345541和MG-132对人脑胶质瘤细胞凋亡及活力的影响  

Effects of protease inhibitors BMS-345541 and MG-132 on cell apoptosis and cell viability of human glioma

作　　者：周保丹 李君 高飞[1] 乔卿均[1] 董辉 ZHOU Baodan;LI Jun;GAO Fei;QIAO Qingjun;DONG Hui(Department of Neurosurgery,Nanyang Second People's Hospital,Nanyang,Henan 473000,China;Department of Neurology,the First Affiliated Hospital of Nanyang Medical College,Nanyang,Henan 473000,China)

机构地区：[1]河南省南阳市第二人民医院神经外科,河南南阳473000 [2]南阳医学高等专科学校第一附属医院神经内科,河南南阳473000

出　　处：《检验医学与临床》2022年第7期868-870,875,共4页Laboratory Medicine and Clinic

基　　金：河南省医学科技攻关计划项目(LHGJ20191471)。

摘　　要：目的研究蛋白酶抑制剂BMS-345541和MG-132对人脑胶质瘤细胞凋亡及活力的影响。方法将15株人脑胶质瘤细胞平均分为对照组、BMS-345541组和MG-132组,每组5株。对照组进行传代培养,BMS-345541组加入BMS-345541进行传代培养,MG-132组加入MG-132进行传代培养。采用MTT比色法检测细胞活力,采用流式细胞仪检测细胞凋亡率,采用荧光定量PCR法检测GRP78 mRNA相对表达量,采用蛋白质免疫印迹法检测蛋白表达水平。结果对照组、BMS-345541组、MG-132组细胞数量分别为(730000±154)、(257000±256)、(210000±333)个,BMS-345541组和MG-132组的细胞数量明显少于对照组,且MG-132组的细胞数量明显少于BMS-345541组,差异有统计学意义(P<0.05);BMS-345541组和MG-132组细胞活力明显低于对照组,细胞凋亡率明显高于对照组,MG-132组的细胞活力高于BMS-345541组,细胞凋亡率低于BMS-345541组,差异有统计学意义(P<0.05);对照组、BMS-345541组、MG-132组的细胞GRP78 mRNA表达水平分别为(2.21±0.08)%、(15.98±1.58)%、(13.55±1.12)%,BMS-345541组和MG-132组的细胞GRP78 mRNA表达水平明显高于对照组,差异有统计学意义(P<0.05);BMS-345541组和MG-132组GRP78、JNK1蛋白表达水平明显高于对照组,差异有统计学意义(P<0.05)。结论蛋白酶抑制剂BMS-345541和MG-132对人脑胶质瘤细胞的凋亡有诱导作用,可增强患者疗效。Objective To investigate the effects of protease inhibitors BMS-345541 and MG-132 on cell apoptosis and cell viability of human glioma.Methods A total of 15 strains of human glioma cells were divided into control group,BMS-345541 group and MG-132 group,5 strains in each group.The control group was subcultured,BMS-345541 group was added with BMS-345541 and subcultured,MG-132 group was added with MG-132 and subcultured.MTT colorimetric assay was used to detect cell viability,cell apoptosis rate,fluorescence quantitative assay was used to detect GRP78 mRNA expression,western blotting was used to detect protein expression.Results The number of cells in the control group,BMS-345541 group and MG-132 group were(730000±154),(257000±256)and(210000±333)respectively,the number of cells in BMS-345541 group and MG-132 group were significantly lower than that in control group,the number of cells in MG-132 group was significantly lower than that in BMS-345541 group(P<0.05).The cell viability of BMS-345541 group and MG-132 group were significantly lower than that of control group,but the cell apoptosis rate of MG-132 group were lower than that of control group(P<0.05).The cell viability in MG-132 group was higher than that in BMS-345541 group,the cell apoptosis rate in MG-132 group was lower than that in BMS-345541 group(P<0.05).The expression of GRP78 mRNA in control group,BMS-345541 group and MG-132 group were(2.21±0.08)%,(15.98±1.58)%,(13.55±1.12)%.The expression of GRP78 mRNA in BMS-345541 group and MG-132 group were significantly higher than that in control group(P<0.05).The relative expressions of GRP78 and JNK1 protein in BMS-345541 group and MG-132 group were significantly higher than those in control group(P<0.05).Conclusion The protease inhibitors BMS-345541 and MG-132 could induce the cell apoptosis of human glioma and enhance patients'efficacy.

关 键 词：BMS-345541 MG-132 人脑胶质瘤 细胞凋亡 细胞活力 

分 类 号：R739.41[医药卫生—肿瘤]