CUL7和CCDC8蛋白在正常小鼠胫骨生长板的表达  

Expression of CUL7 and CCDC8 proteins in normal mouse tibial growth plate

作　　者：付鹏九 张会丰[1] 张亚男[1] Fu Pengjiu;Zhang Huifeng;Zhang Yanan(Department of Pediatrics,Second Hospital of Hebei Medical University,Shijiazhuang 050000,China)

机构地区：[1]河北医科大学第二医院儿科,石家庄050000

出　　处：《中华内分泌代谢杂志》2022年第2期156-160,共5页Chinese Journal of Endocrinology and Metabolism

摘　　要：通过探究CUL7、CCDC8蛋白在0~4周龄正常小鼠胫骨生长板的表达位置变化和表达量的变化,明确CUL7、CCDC8蛋白在正常小鼠胫骨生长板增殖、分化过程中的作用。通过免疫组织化学染色分析CUL7、CCDC8蛋白在0~4周龄正常小鼠胫骨生长板中的表达位置及表达量的变化。CUL7蛋白在0~4周的小鼠胫骨生长板静止带、增殖带和肥大带胞质和胞核中均有表达。CUL7蛋白在生长板各带中的表达量和总表达量,都随着周龄的增加而逐渐减少(F=369.61,P<0.001)。CUL7蛋白的表达量在增殖带下降最为显著,其次是静止带、肥大带。CCDC8蛋白0~2周主要在生长板的增殖带和肥大带胞质和胞核表达,3~4周主要在肥大带胞质和胞核表达。增殖带中的CCDC8蛋白表达量随着周龄的增加而下降,肥大带中的CCDC8蛋白表达量随着周龄的增加而增加(F=453.67,P<0.001)。生长板中总的CCDC8蛋白表达量随着周龄的增加而下降。CUL7、CCDC8蛋白总量都随着周龄增加表达量减少,提示CUL7、CCDC8蛋白可能有促进生长板软骨细胞增殖、分化的作用。To investigate the changes of expression location and amount of CUL7 and CCDC8 proteins in the growth plate of normal mice aged 0-4 weeks,and to clarify the roles of CUL7 and CCDC8 proteins in the proliferation and differentiation of tibia growth plate of mice.The expression location and levels of CUL7 and CCDC8 proteins in the tibial growth plate of normal mice aged 0-4 weeks were observed with immunohistochemical staining.CUL7 protein was expressed in the cell cytoplasm and membrane in three zones of tibial growth plate three bands at 0-4 weeks.The expression level and total expression level of CUL7 protein in each zone of growth plate decreased gradually with the increase of week of age(F=369.61,P=0.001).The expression of CUL7 protein decreased most significantly in the proliferative zone,followed by the stationary zone and the hypertrophic zone.CCDC8 protein was mainly expressed in proliferation zone and hypertrophic zone cell membrane and nuclear membrane of growth plate at 0-2 weeks,and mainly expressed in hypertrophic zone cell membrane and nuclear membrane at 3-4 weeks.The expression of CCDC8 protein in the proliferating zone changed inversely with week of age,and the expression of CCDC8 protein in the hypertrophic zone increased over 4 weeks(F=453.67,P<0.001).The total expression of CCDC8 protein in growth plates decreased with the increase of week of age.The expression levels of CUL7 and CCDC8 decreased with the increase of week of age,suggesting that CUL7 and CCDC8 may promote the proliferation and differentiation of growth plate chondrocytes.

关 键 词：CUL7 CCDC8 生长板 3M综合征 

分 类 号：R336[医药卫生—人体生理学]