菠萝蜜白化突变体叶片表皮形态研究  

Leaf Epidermal Morphology of Albino Mutant of Artocarpus heterophyllus

作　　者：王紫璇 徐芷蕙 黄晓薇 于旭东[1] 蔡泽坪[1] 罗佳佳 李世东[1] WANG Zixuan;XU Zhihui;HUANG Xiaowei;YU Xudong;CAI Zeping;LUO Jiajia;LI Shidong(Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants,Ministry of Education,College of Forestry,Hainan University,Haikou 570228,China;Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China;College of Tropical Crops,Hainan University,Haikou 570228,China)

机构地区：[1]海南大学林学院,热带特色林木花卉遗传与种质创新教育部重点实验室,海口570228 [2]中国热带农业科学院热带作物品种资源研究所,海口571101 [3]海南大学热带作物学院,海口570228

出　　处：《热带亚热带植物学报》2022年第2期249-256,共8页Journal of Tropical and Subtropical Botany

基　　金：海南省自然科学基金项目(319MS017);海南大学科研启动基金项目(kyqd1620);海南大学国家级大学生创新创业训练计划项目(SA2000008501,SA2100001230)资助。

摘　　要：为探究木本植物白化突变体叶片表皮形态的变化,在扫描电镜下观测了菠萝蜜(Artocarpusheterophyllus)白化突变体(AAS)和正常(CK)幼苗叶片的表皮细胞和气孔器,对MAP65家族蛋白构建了进化树,并分析了MAP65基因的表达模式。结果表明,AAS表皮细胞和气孔器的大小、形态均发生较大变化。与CK相比,AAS表皮细胞的周长、面积较小,密度较大,凸出数量和长度均减少,气孔器较小且大小不一。下表皮小细胞和异常气孔器的数量在AAS中大幅增加。MAP65家族成员大部分基因在AAS中下调表达。因此,推测菠萝蜜白化突变体的发生可能与MAP65基因表达有关。To investigate the changes of leaf epidermal morphology in albino mutants of woody plants,the epidermis cells and stomatal apparatus on leaves of albino mutant(AAS)and normal(CK)Artocarpus heterophyllus seedlings were observed under scanning electron microscopy.The phylogenetic tree was constructed based on MAP65 family proteins,and the expression of MAP65 was analyzed.The results showed that the size and morphology of epidermal cells and stomatal apparatus of AAS were changed greatly.Compared with CK,epidermal cells of AAS had smaller perimeter and area,higher density,less lobe number,shorter lobe length and smaller stomatal apparatus.The number of small cells and abnormal stomatal apparatus in lower epidermis increased significantly in AAS.Most genes of MAP65 family members were down regulated in AAS.Therefore,it was speculated that the occurrence of AAS might be related to the expression of MAP65 gene.

关 键 词：菠萝蜜 白化突变体 叶 表皮细胞 气孔器 基因表达 

分 类 号：S667.8[农业科学—果树学]