复方天麻蜜环糖肽片对原发高血压大鼠T淋巴细胞亚群、早期肾损伤及AT1R/Smad3蛋白作用机制研究  

Mechanism of compound Gastrodia honey cyclic glycopeptide tablets in T lymphocyte subsets, early kidney damage and AT1R/Smad3 proteins in essential hypertensive rats

作　　者：冯静 张贤春[1] FENG Jing;ZHANG Xianchun(Haikou People’s Hospital,Haikou 570208,China)

机构地区：[1]海口市人民医院,海口570208

出　　处：《中国比较医学杂志》2022年第3期38-45,共8页Chinese Journal of Comparative Medicine

基　　金：海南省卫生计生行业科研项目(20A200118)。

摘　　要：目的 探究复方天麻蜜环糖肽片对原发性高血压大鼠T淋巴细胞亚群、早期肾损伤及AT1R/Smad3蛋白的作用。方法 本实验共分为3组,正常组(NC组)、模型组(SHR组)和药物组(TMGT组)。分别比较3组大鼠不同时间的血压变化;3组大鼠外周血T淋巴细胞亚群用流式细胞仪进行检测比较;3组大鼠血清中的炎症因子IL-6和TNF-α含量用ELISA进行检测比较;3组大鼠早期肾组织损伤情况用HE染色和PAS染色观察比较;3组大鼠肾组织中AT1R/Smad3蛋白和mRNA表达分别用蛋白质印迹法和RT-PCR法进行检测比较。结果 于给药后2、4和8周时NC组大鼠相比较,SHR组大鼠的尾动脉压升高(P<0.05);和SHR组大鼠相比,TMGT组大鼠的尾静脉收缩压在给药后2、4和8周时得到了显著降低(P<0.05)。SHR组大鼠CD4^(+)/CD8^(+)比值较NC组显著升高(P<0.05);干预后的TMGT组CD4^(+)/CD8^(+)比值明显低于SHR组(P<0.05)。SHR组大鼠血清中IL-6和TNF-α含量较NC组明显升高(P<0.05);和SHR组大鼠血清中IL-6和TNF-α含量相比,TNGT组显著降低(P<0.05)。SHR组大鼠肾组织较NC组损伤严重,肾小球肥大且基底膜增厚,大量肾小球变为空泡,毛细血管狭窄且出现闭塞;干预后的TMGT组大鼠肾组织较SHR组得到了明显的改善。和NC组相比,SHR组大鼠肾组织中Smad3和AT1R蛋白及mRNA表达显著增加(P<0.05);干预后的TMGT组大鼠肾组织中Smad3和AT1R蛋白及mRNA表达较SHR组均显著降低(P<0.05)。结论 复方天麻蜜环糖肽片可有效调节高血压大鼠T淋巴细胞亚群,改善机体的免疫功能,缓解高血压导致的早期肾损伤,同时抑制肾组织中的Smad3和AT1R蛋白表达,进而对高血压疾病进行治疗。Objective To explore the effects of compound Gastrodia honey cyclic glycopeptide tablets on T lymphocyte subsets, early renal injury, and angiotensin II receptor type 1(AT1 R)/Smad3 proteins in essential hypertensive rats. Methods The study was divided into three groups: normal(NC), model(SHR) and drug(TMGT) groups. Changes in blood pressure, T lymphocyte subsets in peripheral blood, IL-6 and TNF-α in serum, pathology in renal tissue, AT1 R/Smad3 protein expression were assessed. Expression of AT1 R/Smad3 mRNA was detected. Results Compared with the NC group, tail artery pressure in the SHR group was increased at 2, 4 and 8 weeks after administration(P<0.05). However, no significant difference was found between SHR and TMGT groups at week 0(P<0.05). At 2, 4 and 8 weeks after administration, systolic blood pressure of the tail artery in the SHR group was significantly higher than that in the NC group, and the tail vein of the TMGT group was systolic after treatment. Blood pressure in the SHR group was significantly lower than that in the SHR group(P<0.05). The ratio of CD4^(+)/CD8^(+)cells in the SHR group was significantly higher than that in the NC group(P<0.05). The ratio of CD4^(+)/CD8^(+)cells in the TMGT group was significantly lower than that in the SHR group after treatment(P<0.05). Compared with the NC group, the serum levels of IL-6 and TNF-α in the SHR group were increased significantly(P<0.05) and those in the TMGT group were decreased significantly(P<0.05). Renal tissue of the SHR group was more seriously damaged than that of the NC group with glomerular hypertrophy and basement membrane thickening, a large number of glomeruli became vacuoles, capillary stenosis and occlusion. After treatment, renal tissue of the TMGT group was significantly improved compared with that of the SHR group. Compared with the NC group, protein and mRNA expression of Smad3 and AT1 R in renal tissue of the SHR group was increased significantly(P<0.05) and that of Smad3 and AT1 R in renal tissue of the TMGT group was decrea

关 键 词：复方天麻蜜环糖肽片 原发性高血压 T淋巴细胞亚群 早期肺损伤 AT1R/Smad3蛋白 

分 类 号：R-33[医药卫生]