海人酸致痫大鼠颞叶、海马区多药耐药基因1、P-糖蛋白的表达及相互关系  

Expression and relationship between MDR1 gene and Pgp in temporal lobe and hippocampus of rats with kainic acid-induced epilepsy

作　　者：晏玉奎[1] 胡建琴[1] 王庄[1] 张冰[1] 钟婧[2] 姜亦珍[3] YAN Yukui;HU Jianqin;WANG Zhuang;ZHANG Bing;ZHONG Jing;JIANG Yizhen(Department of Neurology,Huzhou Central Hospital(Huzhou Normal College Affiliated Central Hospital),Huzhou 313000,China;不详)

机构地区：[1]湖州市中心医院(湖州师范学院附属中心医院)神经内科,313000 [2]湖州市中心医院(湖州师范学院附属中心医院)中心实验室,313000 [3]湖州市中心医院(湖州师范学院附属中心医院)肿瘤科,313000

出　　处：《浙江医学》2022年第5期455-458,469,共5页Zhejiang Medical Journal

基　　金：湖州市科技局公益性应用研究项目(2020GY14)。

摘　　要：目的探讨癫痫大鼠颞叶、海马区多药耐药基因1(MDR1)及P-糖蛋白(Pgp)的表达及其相互关系。方法选择健康清洁级雄性SD大鼠50只。采用随机数字表法将大鼠分为观察组和对照组。观察组大鼠海马区注射海人酸(KA)2μl(含KA 1μg),制作颞叶耐药性癫痫(RE)模型(成功22只),对照组同法注射0.9%氯化钠注射液2μl。两组大鼠饲养、观察1个月后,分别取颞叶、海马区组织,采用免疫组化及实时荧光定量PCR法检测MDR1 mRNA和Pgp表达水平,并分析其相互关系。结果观察组颞叶、海马区的MDR1 mRNA、Pgp表达水平均高于对照组,差异均有统计学意义(均P<0.05)。观察组颞叶、海马区MDR1 mRNA表达水平与Pgp表达水平呈正相关(r=0.55,P<0.05)。结论MDR1、Pgp可能参与RE的发病机制,可为开发相关新的治疗方法或药物提供依据。Objective To investigate the expression and relationship between MDR1 gene and Pgp in temporal lobe and hippocampus of rats with kainic acid-induced epilepsy.Methods A total of fifty SD rats were divided into observation group and control group by random number table method.The rats in the observation group were injected with 2μl KA(including 1μl KA1)in the hippocampus,and the model of drug-resistant epilepsy(RE)in the temporal leaf was established(22 rats were successfully).The rats in the control group were injected with 0.9% sodium chloride injection 2μl.The expression of MDR1 mRNA and Pgp in temporal lobe and hippocampus was detected with real-time quantitative RT-PCR and immunohistochemistry,respectively,after observing for 1 month.Results The expression levels of MDR1 mRNA and Pgp in the temporal lobe and hippocampus in epileptic group were significantly higher than those in control group(all P<0.05),and the expression level of MDR1 mRNA was positively correlated with Pgp(r=0.55,P<0.05).Conclusion MDR1 and Pgp are up-regulated in the temporal lobe and hippocampus of rats with kainic acid-induced epilepsy,suggesting they might be involved in the pathogenesis of drug-resistant epilepsy.

关 键 词：癫痫 多药耐药基因1 P-糖蛋白 颞叶 海马 

分 类 号：R742.1[医药卫生—神经病学与精神病学]