多发性骨髓瘤患者miRNA-21的表达及其临床意义  

Expression of microRNA-21 in multiple myeloma patients and its clinical significances

作　　者：周楠 曹舒兴[2] 王真真 杨琳[1] 刘小军[1] 罗建民[1] Zhou Nan;Cao Shuxing;Wang Zhenzhen;Yang Lin;Liu Xiaojun;Luo Jianmin(Department of Hematology,the Second Hospital of Hebei Medical University,Shijiazhuang 050000,China;Department of Orthopedics,the Second Hospital of Hebei Medical University,Shijiazhuang 050000,China)

机构地区：[1]河北医科大学第二医院血液科,石家庄050000 [2]河北医科大学第二医院骨科,石家庄050000

出　　处：《白血病．淋巴瘤》2022年第2期83-86,共4页Journal of Leukemia & Lymphoma

基　　金：河北省卫生厅科研基金(20190052);河北医科大学第二医院院内基金(2h201833)。

摘　　要：目的探讨多发性骨髓瘤(MM)患者血清中miRNA-21的表达及其意义。方法回顾性分析2019年7月至2020年6月河北医科大学第二医院确诊的55例MM患者以及20名健康对照者资料。MM患者中初诊未治20例,完全缓解(CR)15例,临床复发20例。收集所有受试者血液样本,采用实时荧光定量聚合酶链反应(qRT-PCR)检测miRNA-21的相对表达水平,分析miRNA-21相对表达水平与MM患者β2-微球蛋白(β2-MG)、肌酐、血红蛋白、清蛋白、t(4;14)突变、13q14突变及预后的关系。选择MM细胞株LP-1,采用免疫磁珠分选正常骨髓CD138阳性浆细胞为对照细胞,qRT-PCR检测两组细胞miRNA-21相对表达水平。结果MM组血清miRNA-21的相对表达水平较健康对照组升高(1.50±0.10比1.03±0.06,t=7.04,P=0.002)。初诊未治MM患者和复发难治MM患者血清miRNA-21均高表达(分别为1.50±0.10、3.13±0.32),与健康对照组比较差异均有统计学意义(t值分别为7.04、10.22,均P<0.05)。获得CR的MM患者miRNA-21相对表达水平为1.27±0.25,与健康对照组比较差异无统计学意义(t=1.76,P=0.152)。高β2-MG、高肌酐、低血红蛋白、低清蛋白、有t(4;14)突变、有13q14突变、未获得CR、复发的MM患者血清中miRNA-21相对表达水平均升高(均P<0.05)。LP-1细胞株miRNA-21的相对表达水平较对照细胞升高(1.56±0.05比1.00±0.06),差异有统计学意义(t=11.73,P<0.01)。结论miRNA-21可能是辅助MM诊断及疗效评价的分子标志物。Objective To investigate the expression of miRNA-21 in serum of patients with multiple myeloma(MM)and its significance.Methods The data of 55 MM patients and 20 healthy controls in the Second Hospital of Hebei Medical University from July 2019 to June 2020 were retrospectively analyzed.Among the MM patients,20 cases were diagnosed without treatment,15 cases were in complete remission(CR),and 20 cases were clinically relapsed.Blood samples were collected from all subjects,and the relative expression levels of miRNA-21 were detected by real-time quantitative polymerase chain reaction(qRT-PCR),and the correlation of miRNA-21 expression withβ2-macroglobulin(β2-MG),creatinine,hemoglobin,albumin,t(4;14)mutation,13q14 mutation and prognosis were analyzed.The MM cell line LP-1 was selected,and normal bone marrow CD138-positive plasma cells were sorted by immunomagnetic beads as control cells.The relative expression levels of miRNA-21 in the two groups of cells were detected by qRT-PCR.Results The serum relative expression level of miRNA-21 in MM group was higher than that in healthy control group(1.50±0.10 vs.1.03±0.06,t=7.04,P=0.002).The serum expressions of miRNA-21 in newly diagnosed untreated MM patients and relapsed/refractory MM patients were high(1.50±0.10 and 3.13±0.32),and compared with the healthy control group,the differences were statistically significant(t values were 7.04 and 10.22,both P<0.05).The relative expression level of miRNA-21 in MM patients with complete remission(CR)was 1.27±0.25,which had no significant difference compared with the healthy control group(t=1.76,P=0.152).The serum expression level of miRNA-21 in MM patients with highβ2-MG,high creatinine,low hemoglobin,low albumin,t(4;14)mutation,13q14 mutation,non-remission and recurrence was significantly increased(all P<0.05).The relative expression level of miRNA-21 in LP-1 cell line was higher than that in control cells(1.56±0.05 vs.1.00±0.06),and the difference was statistically significant(t=11.73,P<0.01).Conclusions The miRNA-21

关 键 词：多发性骨髓瘤 微RNAS 血清 生物学标记 

分 类 号：R733.3[医药卫生—肿瘤]