奥拉西坦联合rTMS减轻脑缺血再灌注损伤大鼠缺血侧脑皮质神经元氧化应激损伤  

Oxiracetam combined with rTMS alleviating oxidative stress injury of cerebral cortex neurons on the ischemic side of cerebral ischemia reperfusion injury in rats

作　　者：刘泽晶 徐丰 刘勇[1,3] 吕英 钱晓蕊 杨富佼 赵琳琳 刘曦 LIU Zejing;XU Feng;LIU Yong;LYU Ying;QIAN Xiaorui;YANG Fujiao;ZHAO Linlin;LIU Xi(Heilongjiang University of Traditional Chinese Medicine,Harbin,Heilongjiang 150006,China;The Fourth Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine,Harbin,Heilongjiang 150001,China;The First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine,Harbin,Heilongjiang 150040,China)

机构地区：[1]黑龙江中医药大学,黑龙江省哈尔滨市150006 [2]黑龙江中医药大学附属第四医院,黑龙江省哈尔滨市150001 [3]黑龙江中医药大学附属第一医院,黑龙江省哈尔滨市150040

出　　处：《中南医学科学杂志》2022年第2期162-166,共5页Medical Science Journal of Central South China

基　　金：黑龙江省自然科学基金项目(H2016061);黑龙江省卫生厅科技计划(2020-298);黑龙江省博士后科研项目(LBH-Q16216)。

摘　　要：目的分析奥拉西坦(Oxi)联合重复经颅磁刺激(rTMS)减轻脑缺血再灌注损伤(I/RI)大鼠缺血侧脑皮质神经元氧化应激损伤的机制。方法60只大鼠随机分为对照组、I/RI组、Oxi组、rTMS组和Oxi+rTMS组。比较各组大鼠神经体征缺失评分(NDS)、脑梗死体积和神经元细胞凋亡率,检测缺血侧脑皮质组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、B细胞淋巴瘤2(Bcl-2)、Bcl-2相关X蛋白(Bax)、半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)、活化的Caspase-3(Cleaved Caspase-3)、核因子E2相关因子2(Nrf2)、血红素加氧酶(HO-1)和醌氧化还原酶1(NQO-1)蛋白表达。结果与对照组比较,I/RI组NDS评分和神经元细胞凋亡率升高,SOD和GSH-Px水平下降,MDA水平、Nrf2、HO-1、NQO-1、Cleaved Caspase-3/Caspase-3和Bax/Bcl-2蛋白表达升高(P<0.05)。与I/RI组比较,Oxi组、rTMS组和Oxi+rTMS组NDS评分、脑梗死体积和神经元细胞凋亡率下降,SOD和GSH-Px水平、Nrf2、HO-1、NQO-1蛋白表达升高,MDA水平、Cleaved Caspase-3/Caspase-3和Bax/Bcl-2蛋白表达下降(P<0.05);与Oxi组和rTMS组比较,Oxi+rTMS组上述改变更为显著(P<0.05)。结论Oxi联合rTMS可降低脑I/RI大鼠脑组织氧化应激水平,抑制缺血侧脑皮质神经元细胞凋亡,减轻脑组织氧化应激损伤。Aim To analyze the mechanism of oxiracetam combined with repeated transcranial magnetic stimulation(rTMS)in alleviating oxidative stress injury of cerebral cortical neurons on the ischemic side of cerebral ischemia/reperfusion injury(I/RI)in rats.Methods Sixty SD rats were randomly divided into sham operation group,I/RI group,Oxi group,rTMS group and Oxi+rTMS group.Neurologic sign loss score(NDS),cerebral infarction volume and neuronal cell apoptosis were compared.The levels of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA),B-cell lymphoma 2(Bcl-2),Bcl-2 related X protein(Bax),cysteine aspartic protease 3(Caspase-3),Cleaved Caspase-3,nuclear factor E2 related factor 2(Nrf2),heme oxygenase(HO-1)and quinone oxidoreductase 1(NQO-1)in cerebral cortex tissue onthe ischemic side were detected.Results Compared with sham operation group,NDS and neuronal apoptosis rate were increased in I/RI group,levels of SOD and GSH-Px were decreased,MDA,Nrf2,HO-1,NQO-1,Cleaved Caspase-3/Caspase-3 and Bax/Bcl-2 were increased(P<0.05).Compared with I/RI group,NDS,cerebral infarction volume and neuronal apoptosis rate were decreased in Oxi group,rTMS group and Oxi+rTMS group,levels of SOD,GSH-Px,Nrf2,HO-1 and NQO-1 were increased,MDA,Cleaved Caspase-3/Caspase-3 and Bax/Bcl-2 were decreased(P<0.05).Compared with Oxi group and rTMS group,the above changes were more significant in Oxi+rTMS group(P<0.05).Conclusion Oxi combined with rTMS can reduce the level of oxidative stress in brain tissue of rats with cerebral I/RI,inhibit the apoptosis of cerebral cortex neurons on the ischemic side,and alleviate the injury of oxidative stress in brain tissue.

关 键 词：脑缺血再灌注损伤 奥拉西坦 重复经颅磁刺激 氧化应激 

分 类 号：R743.3[医药卫生—神经病学与精神病学]