Adiporedoxin对TNF-α诱导血管内皮细胞Integrinα4和Integrinβ1表达的影响  

The effect of adiporedoxin on TNF-α-induced Integrinα4 and Integrinβ1 expression in vascular endothelial cells

作　　者：苏波 杨咏梅 刘政海 何慧 苏琦 SU Bo;YANG Yongmei;LIU Zhenghai;HE Hui;SU Qi(Institute of Pharmacy and Pharmacology,School of Pharmacy,University of South China,Hengyang,Hunan 421001,China;Clinical Anatomy and Reproductive Medicine Application Institute,University of South China,Hengyang,Hunan 421001,China;Institute of Cancer,School of Besic Medicine,Hengyang Medical School,University of South China,Hengyang,Hunan 421001,China)

机构地区：[1]南华大学衡阳医学院药学院药物药理研究所,湖南省衡阳市421001 [2]南华大学衡阳医学院基础医学院应用解剖与生殖医学研究所,湖南省衡阳市421001 [3]南华大学衡阳医学院肿瘤研究所,湖南省衡阳市421001

出　　处：《中南医学科学杂志》2022年第2期167-170,共4页Medical Science Journal of Central South China

基　　金：国家自然科学基金(81973532);湖南省自然科学基金(2020JJ4529,2020JJ4522);博士科研启动基金(2018XQD11,2011XQD34)。

摘　　要：目的研究脂过氧化物酶(adiporedoxin,Adrx)对肿瘤坏死因子-α(TNF-α)诱导血管内皮细胞整合素α4(Integrinα4)和整合素β1(Integrinβ1)表达的影响。方法过表达实验分为空载体组、空载体+TNF-α组、Adrx+TNF-α组;si-RNA干扰实验分为si-Control组、TNF-α组、si-Adrx+TNF-α组。将Adrx质粒和si-Adrx分别转染HUVEC细胞后,用TNF-α处理细胞,Real-time PCR和Western blotting分别检测Integrinα4和Integrinβ1 mRNA和蛋白水平。结果TNF-α上调Integrinα4、Integrinβ1表达;与单用TNF-α处理组比较,Adrx+TNF-α组细胞Integrinα4和Integrinβ1 mRNA和蛋白水平均显著降低;而用si-RNA下调Adrx表达后,TNF-α诱导的Integrinα4和Integrinβ1 mRNA和蛋白水平均明显升高。结论Adrx负调控TNF-α诱导的HUVEC细胞Integrinα4和Integrinβ1表达。Aim To investigate the effect of adiporedoxin(Adrx)on Integrinα4 and Integrinβ1 expression induced by tumor necrosis factor-α(TNF-α)in vascular endothelial cells.Methods The overexpression experimental group was divided into vector group,vector+TNF-αgroup,Adrx+TNF-αgroup.si-RNA interference experimental group was divided into si-Control group,TNF-αgroup,si-Adrx+TNF-αgroup.HUVEC cells were transfected with Adrx plasmid and Si-Adrx plasmid,and treated with TNF-α.The mRNA and protein levels of Integrinα4 and Integrinβ1 were detected by real-time PCR and Western blotting,respectively.Results TNF-αupregulated Integrinα4 and Integrinβ1 expression.In contrast to cell treated with TNF-αalone,the mRNA and protein levels of Integrinα4 and Integrinβ1 were obviously decreased in the Adrx+TNF-αgroup.TNF-α-induced Integrinα4 and Integrinβ1 mRNA and protein levels were significantly increased after the expression of Adrx was down-regulated by si-RNA.Conclusion Adrx can negatively modulates Integrinα4 and Integrinβ1 expression induced by TNF-αin HUVEC cells.

关 键 词：脂过氧化物酶 肿瘤坏死因子-α 整合素Α4 整合素Β1 血管内皮细胞 

分 类 号：R364.5[医药卫生—病理学]