骨髓间充质干细胞来源外泌体通过PI3K/Akt途径减轻过氧化氢诱导心肌细胞损伤  

Bone Marrow Mesenchymal Stem Cell Derived Exosomes Attenuate Hydrogen Peroxide Induced Cardiomyocyte Injury via PI3K/Akt Pathway

作　　者：叶莎[1] 杨翠玲 郑媛媛 YE Sha;YANG Cuiling;ZHENG Yuanyuan(Department of Geriatric Cardiology,The Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710000,Shaanxi,China)

机构地区：[1]西安交通大学第二附属医院老年心血管科,陕西西安710000

出　　处：《心血管病学进展》2022年第3期269-273,共5页Advances in Cardiovascular Diseases

基　　金：陕西省重点研发计划(2020FS-254)。

摘　　要：目的研究骨髓间充质干细胞(BMMSC)来源外泌体通过PI3K/Akt途径减轻过氧化氢(H_(2)O_(2))诱导心肌细胞损伤。方法培养心肌H9c2细胞,采用0.5 mmol/L H_(2)O_(2)诱导心肌细胞损伤,给予BMMSC来源外泌体、对照溶剂二甲基亚砜或PI3K抑制剂LY294002干预,检测细胞存活率、凋亡率及Bcl-2、Bax、cleaved caspase-3、p-PI3K和p-Akt的表达水平。结果H_(2)O_(2)组细胞的存活率及Bcl-2、p-PI3K和p-Akt的表达水平低于对照组,凋亡率以及Bax和cleaved caspase-3的表达水平高于对照组(P<0.05);H_(2)O_(2)+外泌体组细胞的存活率及Bcl-2、p-PI3K和p-Akt的表达水平高于H_(2)O_(2)组,凋亡率以及Bax和cleaved caspase-3的表达水平低于H_(2)O_(2)组(P<0.05);LY294002+H_(2)O_(2)+外泌体组细胞的存活率以及Bcl-2、p-PI3K和p-Akt的表达水平低于溶剂对照+H_(2)O_(2)+外泌体组,凋亡率以及Bax和cleaved caspase-3的表达水平高于溶剂对照+H_(2)O_(2)+外泌体组(P<0.05)。结论BMMSC来源外泌体通过激活PI3K/Akt途径减轻H_(2)O_(2)诱导心肌细胞损伤。Objective To study the effect of bone marrow mesenchymal stem cell(BMMSC)derived exosomes attenuate hydrogen peroxide(H_(2)O_(2))induced cardiomyocyte injury via PI3K/Akt pathway.Methods H9c2 cells were cultured,0.5 mmol/L H_(2)O_(2) was used to induce cardiomyocyte injury.BMMSC derived exosomes,DMSO or PI3K inhibitor LY294002 were used to intervene.The cell survival rate,apoptosis rate and the expression levels of Bcl-2,Bax,cleaved caspase-3,p-PI3K and p-Akt were detected.Results The cell survival rate and the expression levels of Bcl-2,p-PI3K and p-Akt of H_(2)O_(2) group were lower than those of control group,while the apoptosis rate and the expression levels of Bax and cleaved caspase-3 were higher than those of control group(P<0.05).The cell survival rate and the expression levels of Bcl-2,p-PI3K and p-Akt of H_(2)O_(2)+exosome group were higher than those of H_(2)O_(2) group,while the apoptosis rate and the expression levels of Bax and cleaved caspase-3 were lower than those of H_(2)O_(2) group(P<0.05).The cell survival rate and the expression levels of Bcl-2,p-PI3K and p-Akt of LY294002+H_(2)O_(2)+exosomes group were lower than those of solvent control+H_(2)O_(2)+exosomes group,while the apoptosis rate and the expression levels of Bax and cleaved caspase-3 were higher than those of solvent control+H_(2)O_(2)+exosomes group(P<0.05).Conclusion BMMSC derived exosomes attenuate H_(2)O_(2) induced cardiomyocyte injury by activating PI3K/Akt pathway.

关 键 词：骨髓间充质干细胞 外泌体 过氧化氢 凋亡 PI3K/AKT途径 

分 类 号：R73[医药卫生—肿瘤]