DEVELOPMENT OF NEEDLE-BASED MICROENDOSCOPY FOR FLUORESCENCE MOLECULAR IMAGING OF BREAST TUMOR MODELS  

作　　者：CHAO-WEI CHEN TIFFANY R.BLACKWELL RENEE NAPHAS PAUL T.WINNARD JR VENU RAMAN KRISTINE GLUNDE YU CHEN 

机构地区：[1]Fischell Department of Bioengineering and Electrical and Computer Engineering,University of Maryland College Park,MD 20742,USA [2]In Vivo Cellular and Molecular Imaging Center(ICMIC)Department of Radiology,Johns Hopkins University School of Medicine Baltimore,MD 21205,USA§[e-mail]¶[e-mail]

出　　处：《Journal of Innovative Optical Health Sciences》2009年第4期343-352,共10页创新光学健康科学杂志（英文）

基　　金：the Nano-Biotechnology Award of the State of Maryland,the Minta Martin Foundation,the General Research Board(GRB)Award of the University of Maryland,and the University of Maryland Baltimore(UMB)and College Park(UMCP)Seed Grant Program,and the Prevent Cancer Foundation(to Y.C.);Support from NIH P50 CA103175(JHU ICMIC Program,to V.R.);NIH CA134695(to K.G.)is gratefully acknowledged.

摘　　要：Fluorescence molecular imaging enables the visualization of basic molecular processes such as gene expression,enzyme activity,and disease-specific molecular interactions in vivo using targeted contrast agents,and therefore,is being developed for early detection and in situ characterization of breast cancers.Recent advances in developing near-infrared fluorescent imaging contrast agents have enabled the specific labeling of human breast cancer cells in mouse model systems.In synergy with contrast agent development,this paper describes a needle-based fluorescence molecular imaging device that has the strong potential to be translated into clinical breast biopsy procedures.This microendoscopy probe is based on a gradient-index(GRIN)lens interfaced with a laser scanning microscope.Specifications of the imaging performance,including the field-of-view,transverse resolution,and focus tracking characteristics were calibrated.Orthotopic MDA-MB-231 breast cancer xenografts stably expressing the tdTomato red fluorescent protein(RFP)were used to detect the tumor cells in this tumor model as a proof of principle study.With further development,this technology,in conjunction with the development of clinically applicable,injectable fluorescent molecular imaging agents,promises to perform fluorescence molecular imaging of breast cancers in vivo for breast biopsy guidance.

关 键 词：Breast cancer fluorescence molecular imaging MICROENDOSCOPY optical imaging tdTomato fluorescent protein. 

分 类 号：R73[医药卫生—肿瘤]