东方蜜蜂微孢子虫蓖麻毒素B凝集素基因的分子克隆及生物信息学分析  

Molecular cloning and bioinformatic analysis of Nosema ceranaericin B-lectin gene

作　　者：范元婵 王杰 孙明会 吴鹰 余岢骏 王心蕊 叶亚萍 钱加珺 张凯遥 王思懿 陈大福 郭睿 FAN Yuanchan;WANG Jie;SUN Minghui;WU Ying;YU Kejun;WANG Xinrui;YE Yaping;QIAN Jiajun;ZHANG Kaiyao;WANG Siyi;CHEN Dafu;GUO Rui(College of Animal Sciences(College of Bee Science),Fujian Agriculture and Forestry University,Fuzhou 350002,China;Apitherapy Research Institute,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学动物科学学院(蜂学学院),福州350002 [2]福建农林大学蜂疗研究所,福州350002

出　　处：《中国农业大学学报》2022年第3期138-145,共8页Journal of China Agricultural University

基　　金：国家现代农业产业技术体系建设专项资金(CARS-44-KXJ7);福建农林大学杰出青年科研人才计划项目(xjq201814);江西省蜜蜂生物学与饲养重点实验室开放基金项目(JXKLHBB-2020-04)。

摘　　要：为获得东方蜜蜂微孢子虫(Nosema ceranae)的NCRBL-081基因序列,并对NCRBL-081编码蛋白进行预测和分析。本研究利用RT-PCR克隆NCRBL-081基因,利用相关生物信息学工具对NCRBL-081编码蛋白进行预测。结果表明:NCRBL-081共含有630个核苷酸,可编码含209个氨基酸的亲水性蛋白;NCRBL-081编码蛋白含有1个信号肽,6个明显的丝氨酸,5个酪氨酸磷酸化位点及1个跨膜结构域;该蛋白含有20.10%的α螺旋,8.61%的β折叠,25.84%的延伸链及45.45%的无规卷曲,且具有Ⅱ型核糖体失活蛋白家族的典型特征;同一个微孢子虫物种的不同RBL蛋白之间进化距离较近,而不同微孢子虫物种的RBL蛋白之间进化距离较远;东方蜜蜂微孢子虫的NCER;00581与AAJ76;00008081之间亲缘关系较近。研究结果解析了东方蜜蜂微孢子虫NCRBL-081的序列,并揭示了东方蜜蜂微孢子虫NCRBL-081编码蛋白的分子特征,可为后续功能探究提供依据。The aims of this study are to obtain the sequence of Nosema ceranae NCRBL-081 gene and predict and analyze the NCRBL-081 coding protein.NCRBL-081 gene is cloned by RT-PCR.The encoded protein of NCRBL-081 gene is predicted by related bioinformatics tools.The results indicate that:NCRBL-081 contains 630 nucleotides encoding a hydrophilic protein of 209 amino acids;NCRBL-081 encoded protein cis consisted of one signal peptide,six serine phosphorylation sites,five tyrosine phosphorylation sites and one transmembrane domain;Additionally,the encoded protein has 20.10% of alpha helix,8.61% of beta fold,25.84% of extended chain and 45.45% of irregular coil,and it has typical characteristics of type II ribosome inactivating protein family;Various RBL proteins from the same microsporidian species display closer evolutionary distance,while RBL proteins from different microsporidian species have more distant evolutionary distance;Moreover,NCER_100581 and AAJ76_100008081 in N.ceranae have a closer genetic relationship.The study obtained the full sequence of NCRBL-081 in N.ceranae and revealed the molecular characteristics of NCRBL-081 coding protein,which lay a foundation for the following functional analyses.

关 键 词：东方蜜蜂微孢子虫 蓖麻毒素B凝集素 分子克隆 生物信息学 系统进化树 

分 类 号：S891[农业科学—特种经济动物饲养]