水杨酸纳米乳的制备、体外释放行为及抑菌活性研究  

Preparation, in vitro release behavior and antibacterial activity of salicylic acid nanoemulsion

作　　者：陈家铃 吴航航 何益飞 鲁旺旺 刘倩 王曦 罗丹[1,2] 刘卫 CHEN Jia-ling;WU Hang-hang;HE Yi-fei;LU Wang-wang;LIU Qian;WANG Xi;LUO Dan;LIU Wei(Wuhan Bestcarrier Biotechnology Co.,Ltd.,Wuhan 430075,China;National Engineering Research Center for Nanomedicine,Huazhong University of Science and Technology,Wuhan 430075,China;Guangzhou Jiyan Cosmetics Technology Co.,Ltd.,Guangzhou 510275,China;College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,China)

机构地区：[1]武汉百思凯瑞生物科技有限公司,武汉430075 [2]华中科技大学国家纳米药物工程技术研究中心,武汉430075 [3]广州集妍化妆品科技有限公司,广州510275 [4]华中科技大学生命科学与技术学院,武汉430074

出　　处：《精细与专用化学品》2022年第3期16-21,共6页Fine and Specialty Chemicals

摘　　要：通过纳米载体技术将高浓度水杨酸进行包载制备成纳米乳,对其理化性质、稳定性、体外释放行为、安全性及抑菌活性进行评价。所制备水杨酸纳米乳形态均一、平均粒径为21.4nm,多分散系数(polydispersity, PDI)为0.107,载药量为30.0%,包封率为83.7%;在不同条件稳定性考察30d后,外观及平均粒径、PDI未发生明显变化,显示纳米乳稳定性良好;体外释放实验显示,水杨酸纳米乳具有缓释和控释特征,不同pH对水杨酸纳米乳释放行为存在一定影响,而脂肪酶能加快水杨酸纳米乳的释放;HET-CAT试验及人体斑贴试验结果显示,水杨酸纳米乳安全性良好;抑菌实验显示,与游离水杨酸比较,制备的水杨酸纳米乳能显著抑制痤疮丙酸杆菌的活性。Nano-emulsion was prepared by encapsulating high-concentration salicylic acid through nano-carrier technology.The physical and chemical properties, stability, in vitro release behavior, safety and antibacterial activity were evaluated.The prepared salicylic acid nanoemulsion has a uniform shape, an average particle size of 21.4 nm, and a polydispersity(PDI) of 0.107,the drug loading was 30.0%,and the encapsulation rate was 83.7%.After being placed under different conditions for 30 days, the appearance, average particle size, and PDI have not changed significantly, and the nanoemulsion has good stability.In vitro release experiments showed that the salicylic acid nanoemulsion has sustained and controlled release characteristics.Different pH has certain influence on the release behavior of salicylic acid nanoemulsion, and lipase can accelerate the release of salicylic acid nanoemulsion.HET-CAT test and human patch test results showed that salicylic acid nanoemulsion was safe.Bacteriostatic experiments showed that compared with free salicylic acid, the prepared salicylic acid nanoemulsion can significantly inhibit the activity of P.acnes.

关 键 词：水杨酸 纳米乳 释放行为 安全性 抑菌活性 

分 类 号：TQ465[化学工程—制药化工]