氨基酸/氧化石墨烯/羟基磷灰石复合材料在酸蚀人牙釉质体外再矿化中的应用  

Amino Acids/Graphene Oxide/Hydroxyapatite Composites on in vitro Remineralization of Acid-Etched Human Enamel

作　　者：蔡英 钏定泽 陈庆华[2] 刘继涛 CAI Ying;CHUAN Dingze;CHEN Qinghua;LIU Jitao(Yunnan Baiyao Group Health Products Co., Ltd., Kunming 650217, China;Faculty of Materials Science and Engineering, Kunming University of Science and Technology, Kunming 650093, China)

机构地区：[1]云南白药集团健康产品有限公司,昆明650217 [2]昆明理工大学材料科学与工程学院,昆明650093

出　　处：《人工晶体学报》2022年第3期516-522,共7页Journal of Synthetic Crystals

摘　　要：本文研究了甘氨酸(Gly)和氧化石墨烯(GO)复合改性羟基磷灰石(Gly/GO/HA),以及天冬氨酸(Asp)和GO复合改性羟基磷灰石(Asp/GO/HA)对酸蚀人牙釉质(HE)体外再矿化的影响。通过CCK-8法测定不同复合材料的细胞毒性,通过场发射扫描电镜、EDX能谱分析、X射线衍射和显微硬度测试,对再矿化前/后HE表面及剖面形貌、表面元素组成、表面成分结构和表面力学性能进行表征。结果表明:Gly/GO/HA和Asp/GO/HA均有良好的安全性和生物相容性,且均能使酸蚀HE得到一定的修复,其中Gly/GO/HA对酸蚀HE表面和深层脱矿的修复效果优于Asp/GO/HA。Effects of glycine(Gly)and graphene oxide(GO)modified hydroxyapatite(Gly/GO/HA),and aspartic acid(Asp)and GO modified hydroxyapatite(Asp/GO/HA)on in vitro remineralization of acid-etched human enamel(HE)were investigated.The cytotoxicity of these composites was studied by CCK-8 method,the surface and cross-section morphology,surface element composition,surface composition structure and surface mechanical properties of HE before and after remineralization were characterized by field emission scanning electron microscope,EDX energy spectrum analysis,X-ray diffraction and microhardness test.The results show that Gly/GO/HA and Asp/GO/HA both have good safety and biocompatibility,and both can repair the acid-etched HE to a certain extent,among which Gly/GO/HA is better than Asp/GO/HA in repairing the surface and deep demineralization of the acid-etched HE.

关 键 词：氨基酸 氧化石墨烯 羟基磷灰石 牙釉质 再矿化 细胞毒性 

分 类 号：R318.08[医药卫生—生物医学工程] TB383.1[医药卫生—基础医学]