钛表面纳米管结构金纳米颗粒加载对成骨细胞MC3T3-E1功能影响的研究  

Gold nanoparticle loaded nanotube structure on titanium surface promotes osteogenic differentiation of preosteoblasts

作　　者：徐博雅 宋文 何奕德 李哲 张玉梅 XU Boya;SONG Wen;HE Yide;LI Zhe;ZHANG Yumei(State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi Key Laboratory of Stomatology,Department of Prosthodontics,School of Stomatology,The Fourth Military Medical University,China,Xi'an 710032;Department of Implant Dentistry,College of Stomatology,Xi'an Jiaotong University)

机构地区：[1]军事口腔医学国家重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔医学重点实验室,第四军医大学口腔医院修复科,西安710032 [2]西安交通大学口腔医院种植科

出　　处：《实用口腔医学杂志》2022年第2期184-188,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:81530051)。

摘　　要：目的:在钛纳米管表面加载金纳米颗粒并探究对成骨细胞成骨分化的影响。方法:本研究使用直流电沉积技术将金纳米颗粒沉积在二氧化钛纳米管阵列表面。场发射扫描电子显微镜进行材料表征,CCK-8实验检测细胞增殖能力,通过RT-PCR、ALP染色、茜素红染色检测小鼠成骨细胞MC3T3-E1在改性材料表面成骨分化能力。结果:通过直流电沉积技术成功将金纳米粒子成功组装在二氧化钛纳米管表面,改变电沉积时间可以控制金颗粒的尺寸和负载量。体外实验结果表明,金纳米粒子的加入增加MC3T3成骨分化特异性基因Osx、BMP-2和OPN的表达(P<0.05),显著增强ALP活性和矿化能力(P<0.01)。结论:钛纳米管结构加载金纳米颗粒可以促进MC3T3细胞成骨分化。Objective:To modify the surface of titanium nanotubes with gold nanoparticles and to investigate the effects of the modied surface on the osteogenic differentiation of preosteoblasts.Methods:Gold nanoparticles(AuNPs)were deposited on the surface of titanium dioxide nanotube arrays using direct current deposition technique.Field emission scanning electron microscopy was used for material characterization.Mouse preosteoblasts MC3T3 cells were cultured on the material surface,CCK-8 assay was used to detect cell proliferation.Cell osteogenic differentiation was examined by RT-PCR,ALP staining and alizarin red staining.Results:AuNPs were successfully assembled on titanium dioxide nanotube arrays by direct current deposition technique,and the size and the amount of the loaded AuNPs could be controlled by changing the electrodeposition time.The addition of AuNPs increased the proliferation and the expression of osteogenic differentiation genes Osx,BMP-2 and OPN(P<0.05),enhanced ALP activity and mineralization ability of MC3T3 cells(P<0.01).Conclusion:The titanium nanotube structures loaded with gold nanoparticles can promote osteogenic differentiation of preosteoblasts.

关 键 词：二氧化钛纳米管阵列 金纳米粒子 电沉积技术 成骨分化 

分 类 号：R783.6[医药卫生—口腔医学]