骨化三醇通过NF-κB信号通路促进牙周膜成纤维细胞分化  

Calcitriol promotes the differentiation of periodontal ligament fibroblasts through NF-κB signaling pathway

作　　者：石金双 刘金艳 肖树林 张江琳 李晨 SHI Jinshuang;LIU Jinyan;XIAO Shulin;ZHANG Jianglin;LI Chen(Department of Periodontal Mucosa,Yinchuan Stomatological Hospital,China,750001;Department of Orthodontics,Yinchuan Stomatological Hospital,China,750001)

机构地区：[1]银川市口腔医院牙周黏膜科,750001 [2]银川市口腔医院正畸科,750001

出　　处：《实用口腔医学杂志》2022年第2期265-268,共4页Journal of Practical Stomatology

摘　　要：从青少年正畸拔除的前磨牙牙周组织中培养人牙周膜成纤维细胞(HPDLFs),不同浓度VD 3处理后检测细胞增殖情况、免疫荧光染色细胞角蛋白、波形丝蛋白用于鉴定细胞。细胞培养中添加NF-κB抑制剂、NF-κB激活剂,检测ALP活性、细胞矿化、细胞中ALP、RUNX2、OCN mRNA水平、细胞胞核中NF-κB p65蛋白水平。结果表明,VD 3通过抑制NF-κB入核抑制细胞增殖、促进细胞ALP活性和RUNX2、OCN mRNA表达,降低细胞核中NF-κB p65蛋白水平,促进细胞矿化。Human periodontal ligment fibroblasts(HPDLFs)were cultured from the periodontal tissues of the premolars extracted from teenagers.Immunofluorescence staining of cytokeratin and vimentin proteins was used to identify the cells.The cell proliferation was tested after treatment by VD 3 with various concentritions.NF-κB inhibitor and NF-κB activator was respectively used in the cultures and ALP activity,cell mineralization and mRNA level of RUNX2,OCN,cell nuclei NF-κB p65 protein level were tested.Experimental results showed that VD 3 inhibited cell proliferation,reduced cell ALP activity and mRNA expression of RUNX2,OCN mRNA levels by inhibiting NF-5κB from entering the cell nucleus,reduced the level of NF-κB p65 protein in the nucleus,and promoted cell mineralization.

关 键 词：骨化三醇 核转录因子kappa B信号通路 人牙周膜成纤维细胞 增殖 分化 

分 类 号：R781.4[医药卫生—口腔医学]