抑制分离缺陷基因3表达的宫颈癌细胞增殖、凋亡、侵袭及迁移能力观察  

Proliferation,apoptosis,invasion and migration of cervical cancer cells with inhibited expression of PARD3

作　　者：刘迎嘉[1] LIU Yingjia(Department of Pathology,The Fifth Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学第五附属医院病理科,乌鲁木齐830011

出　　处：《山东医药》2022年第6期1-5,共5页Shandong Medical Journal

基　　金：新疆维吾尔自治区自然科学基金资助项目(2017D01C271)。

摘　　要：目的观察抑制分离缺陷基因3(PARD 3)表达的宫颈癌细胞系SiHa增殖、凋亡、侵袭及迁移能力变化,并探讨其可能作用机制。方法取对数生长期SiHa细胞分为siRNC组、siRNA1组及siRNA2组,分别用骨架质粒、PARD 3siRNA1(抑制PARD3基因表达)、PARD 3siRNA2(抑制PARD3基因表达)腺病毒真核载体转染。采用qRTPCR法检测三组转染后细胞PARD3、JAK2 mRNA。培养24、48 h时采用WesternBlotting法检测三组PARD3蛋白,培养48 h时检测三组细胞JAK2、p-JAK2蛋白。采用CCK-8法观察三组细胞增殖情况,细胞凋亡检测试剂盒检测细胞凋亡情况。培养48 h时取各组细胞,采用Transwell小室观察细胞迁移、侵袭情况。结果与siRNC组比较,siR⁃NA1组、siRNA2组PARD3 mRNA相对表达量低(P均<0.05);与siRNA1组比较,siRNA2组PARD3 mRNA相对表达量低(P<0.05)。与siRNC组比较,培养48 h时siRNA1组、siRNA2组p-JAK2蛋白相对表达量均增加(P均<0.05)。与siRNC组比较,培养24、48 h时siRNA1组、siRNA2组细胞的OD450值增加(P均<0.05)。与siRNC组比较,siRNA2组细胞凋亡率降低(P<0.05)。与siRNC组比较,培养48 h时siRNA1组、siRNA2组细胞迁移数、侵袭数增加(P均<0.05)。结论抑制PARD3表达的SiHa细胞细胞增殖能力升高、细胞凋亡能力降低、侵袭迁移能力增强。PARD3可能通过上调p-JAK2表达促进SiHa细胞的增殖、侵袭及迁移,抑制细胞凋亡。Objective To observe the changes of proliferation,apoptosis,invasion and migration of cervical cancer cells with inhibited expression of PARD3,and to explore its mechanism.Methods SiHa cells in the logarithmic growth phase were divided into the siRNC group,siRNA1 group,and siRNA2 group.Cells in the siRNA1 group and siRNA2 group were transfected with adenovirus eukaryotic vectors of skeleton plasmid PARD 3siRNA1(PARD3 gene silencing)and PARD3siRNA2(PARD3 gene silencing),respectively.qRT-PCR was used to detect PARD3 and JAK2 mRNA.Western blotting was used to detect PARD3 protein in the three groups at 24 and 48 h as well as JAK2 and p-JAK2 protein in the three groups at 48 h.Cell proliferation was observed by CCK-8 and apoptosis was detected by Annexinv-FITC/PI apoptosis detection kit.After 48 h of culture,cells of each group were taken,and cell migration and invasion were ob⁃served in the transwell chamber.Results Compared with the siRNC group,the relative expression levels of PARD3 mRNA in the siRNA1 and siRNA2 groups were lower(both P<0.05).Compared with the siRNA1 group,the rela⁃tive expression level of PARD3 mRNA was lower in the siRNA2 group(P<0.05).Compared with the siRNC group,the relative expression levels of P-JAK2 protein increased in the siRNA1 and siRNA2 groups at 48 h of culture(both P<0.05).Compared with the siRNC group,OD450 value of the siRNA1 group and siRNA2 group increased at 24 and 48 h of culture(all P<0.05).Compared with the siRNC group,the apoptosis rate of the siRNA2 group was lower(P<0.05).Compared with the siRNC group,the numbers of migration and invasion cells increased in the siRNA1 and siRNA2 groups at 48 h of culture(all P<0.05).Conclusions Inhibition of PARD3 expression increases the proliferation,invasion and migration,and decreases the apoptosis of SiHa cells.PARD3 may promote proliferation,invasion and migration of Si⁃Ha cells and inhibit apoptosis by up-regulating p-JAK2 expression.

关 键 词：分离缺陷基因3 磷酸化酪氨酸激酶2 基因沉默 宫颈肿瘤 宫颈癌 细胞增殖 细胞凋亡 细胞侵袭 细胞迁移 

分 类 号：R365[医药卫生—病理学]