IGF-1R通过调控JAK/STAT信号通路对脓毒症大鼠作用机制的研究  

Mechanism of IGF-1R on Sepsis Rats by Regulating JAK/STAT Signaling Pathway

作　　者：胡伟[1] 张涛[1] 盛尚春[1] 刘翔[1] 丁贵梅 代琼[1] HU Wei;ZHANG Tao;SHENG Shang-chun;LIU Xiang;DING Gui-mei;DAI Qiong(Department of Clinical Laboratory,the Second People’s Hospital of Yibin,Sichuan Yibin 644000,China)

机构地区：[1]宜宾市第二人民医院检验科,四川宜宾644000

出　　处：《现代检验医学杂志》2022年第2期6-11,共6页Journal of Modern Laboratory Medicine

基　　金：国家自然科学基金委员会资助项目(B1702101)。

摘　　要：目的 探究1型胰岛素样生长因子受体(insulin-like growth factor 1 receptor,IGF-1R)通过调控Janus激酶/信号转导和转录激活因子(janus kinase/signal transducer and activvator of transcription,JAK/STAT)信号通路对脓毒症大鼠作用机制。方法 将60只实验大鼠随机分为假手术组(sham组)、脓毒症大鼠模型组(sepsis组)和脓毒症大鼠模型给予IGF-1R干预组(IGF-1R组),每组20只。检测肺组织中IGF-1R阳性细胞比例(免疫荧光染色法),检测血清中IL-6和TNF-α含量(ELISA法),检测肺组织病理学改变(HE染色法),TUNEL检测各组细胞凋亡率,免疫印迹法检测组织中p-JAK1和p-STAT3蛋白表达。结果 组织中IGF-1R阳性细胞在sham组(58.32%±5.21%),sepsis组(21.65%±8.73%)和IGF-1R组(46.58%±6.72%)占比比较差异有统计学意义(F=141.7,P<0.000 1);和sham组相比,sepsis组大鼠肺组织中IGF-1R阳性细胞在总细胞中所占比例减少(t=16.13,P<0.000 1),IGF-1R组大鼠肺组织中IGF-1R阳性细胞在总细胞中所占比例高于sepsis组(t=10.12,P<0.000 1),差异均有统计学意义。血清中IL-6在sham组(4.35±2.01pg/ml)、sepsis组(9.81±4.26pg/ml)和IGF-1R组(5.11±2.57pg/ml)中的含量比较差异有统计学意义(F=18.23,P<0.000 1),血清中TNF-α含量在sham组(2.31±1.12pg/ml)、sepsis组(14.15±3.26pg/ml)和IGF-1R组(6.58±2.15pg/ml)比较差异有统计学意义(F=130.7,P<0.000 1),和sham组相比,sepsis组IL-6和TNF-α含量升高(t=5.18,21.98,均P<0.000 1);IGF-1R组IL-6和TNF-α含量较sepsis组降低,差异具有统计学意义(t=4.23,12.01,均P<0.000 1)。细胞凋亡率在sham组(6.35%±2.51%)、sepsis组(37.84%±6.62%)和IGF-1R组(22.71%±4.28%)间比较差异有统计学意义(F=217.4,P<0.000 1)。和sham组相比,sepsis组细胞凋亡率增多(t=19.89,P<0.000 1);和sepsisi组相比,IGF-1R组大鼠肺组织中凋亡率降低(t=8.583,P<0.000 1)。肺组织中p-JAK1在sham组(0.15±0.11)、sepsis组(0.33±0.16)和IGF-1R组(0.17±0.13)中表达差异有统计学意义(F=10.70,P=0.000 1)。�Objective To explore the mechanism of insulin-like growth factor 1 receptor(IGF-1 R) on sepsis rats by regulating, janus kinase/signal transducer and activvator of transcription(JAK/STAT) signa pathway. Methods Sixty experimental rats were randomly divided into sham operation group(sham group), sepsis rat model group(sepsis group) and sepsis rat model group given IGF-1 R intervention group(IGF-1 R group), with 20 rats in each group. The proportion of IGF-1 R positive cells in rat lung tissue was detected by immunofuorescence staining. The contents of IL-6 and TNF-α in serum were detected by ELISA. The pathological changes of lung tissue were detected by HE staining. Cell apoptosis in each group was detected by TUNEL. The protein expression of p-JAK1 and p-STAT3 in tissues was detected by Western blotting. Results The proportion of IGF-1 R positive cells in the sham group(58.32%±5.21%), sepsis group(21.65%±8.73%) and IGF-1 R group(46.58%±6.72%) was statistically significant(F=141.7, P<0.000 1).Compared with the sham group, the number of IGF-1 R positive cells in the sepsis group was decreased(t=16.13, P<0.000 1), and the number of IGF-1 R positive cells in the sepsis group was higher than that in the sepsis group(t=10.12, P<0.000 1), the differences were statistically significant, respectively. The serum IL-6 levels in the sham group(4.35±2.01 pg/ml), the sepsis group(9.81±4.26 pg/ml) and the IGF-1 R group(5.11±2.57 pg/ml) were statistically significant different(F=18.23, P<0.000 1). The serum TNF-α levels in the sham group(2.31±1.12 pg/ml), sepsis group(14.15±3.26 pg/ml) and IGF-1 R group(6.58±2.15 pg/ml) were significantly different(F=130.7, P<0.000 1). Compared with the sham group, IL-6 and TNF-α increased in the sepsis group(t=5.18, 21.98, all P<0.000 1). The levels of IL-6 and TNF-α in IGF-1 R group were lower than those in sepsis group(t=4.23, 12.01, all P<0.000 1). The cell apoptosis rate was significantly different between the sham group(6.35%±2.51%), the sepsis group(37.84±6.62%) and the IG

关 键 词：1型胰岛素样生长因子受体 Janus激酶/信号转导和转录激活因子信号通路 脓毒症 

分 类 号：R-332[医药卫生]