正十六烷降解菌的分离、鉴定及降解特性研究  

Isolation, identification and degradation characteristics of n-hexadecane degrading bacteria

作　　者：李迎鹤 刘东 于杰[1] 赵平南 张潇元 苏俊涛 韩松[1] LI Ying-he;LIU Dong;YU Jie;ZHAO Ping-nan;ZHANG Xiao-yuan;SU Jun-tao;HAN Song(College of Forestry,Northeast Forestry University,Harbin 150040,China;Jilin Branch of China Kunlun Engineering Co.,Ltd.,Jilin 132000,China)

机构地区：[1]东北林业大学林学院,哈尔滨150040 [2]中国昆仑工程有限公司吉林分公司,吉林吉林132000

出　　处：《哈尔滨商业大学学报（自然科学版）》2022年第2期131-136,共6页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：中国昆仑工程有限公司吉林分公司炼化企业纳污水体治理技术研究项目(YL5702000-Y-001)。

摘　　要：以持续供给正十六烷为外界环境压力,从大庆石油污染土壤中筛选出一株正十六烷高效降解菌株,命名为L1.经菌株形态特征观察和16S rDNA基因测序,确定该降解菌株为溶血不动杆菌(Acinetobacter haemolyticus).通过单因素试验探究环境因子(正十六烷体积分数、菌液接种量、初始pH值、NaCl质量浓度、培养时间)对该菌株降解正十六烷能力的影响.研究结果表明,该菌株在接种量4%、初始pH值8、NaCl质量浓度5 g/L,35℃,150 r/min振荡培养的条件下,12 d后对体积分数为0.5%(3867 mg/L)的正十六烷降解率可达62.0%.In this paper, the continuous supply of n-hexadecane was taken as the external environmental pressure, and a strain of n-hexadecane degrading efficiently was selected from Daqing petroleum-contaminated soil and named L1. Observation of the morphological characteristics of the strain and 16 S rDNA gene sequencing confirmed that the degrading strain was Acinetobacter haemolyticus. A single factor experiment was conducted to explore the effects of environmental factors(n-hexadecane volume fraction, inoculum amount, initial pH, NaCl mass concentration, culture time) on the ability of the strain to degrade n-hexadecane. The results of the study showed that the strain had an inoculum volume of 4%, initial pH value of 8, NaCl mass concentration of 5 g/L, 35 ℃, and 150 r/min shaking culture conditions, The degradation rate of n-hexadecane with volume fraction of 0.5%(3 867 mg/L) reached 62.0% after 12 days.

关 键 词：石油污染 正十六烷 降解菌 菌种鉴定 影响因素 降解率 

分 类 号：X172[环境科学与工程—环境科学]