高通量测序分析PIK3CA突变型与野生型结直肠癌的肠道菌群特征  

Microbial characteristics in PIK3CA-mutant and-wild-type colorectal cancer based on high-throughput sequencing

作　　者：尚付梅 任中海[1] 万里新[1] 张敬伟[1] 李长生[1] 马慧利[1] 张蕾[1] 冀叶[1] 袁小笋 刘红利[2] Fumei Shang;Zhonghai Ren;Lixin Wan;Jingwei Zhang;Changsheng Li;Huili Ma;Lei Zhang;Ye Ji;Xiaosun Yuan;Hongli Liu(Department of Medical Oncology,Nanyang Central Hospital,Nanyang 473000,China;Cancer Center,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China)

机构地区：[1]南阳市中心医院肿瘤内科,河南省南阳市473000 [2]华中科技大学同济医学院附属协和医院肿瘤中心腹部肿瘤科

出　　处：《中国肿瘤临床》2022年第6期280-285,共6页Chinese Journal of Clinical Oncology

基　　金：国家重点研发计划项目(编号:2018YFC1313300);吴阶平医学基金会医学科研专项基金项目(编号:320.6750.2021-02-126)资助。

摘　　要：目的:采用16S rDNA二代高通量测序技术,研究PIK3CA突变型与野生型结直肠癌患者中的肠道菌群特征。方法:选取2016年12月至2017年12月华中科技大学同济医学院附属协和医院结直肠癌患者19例,采用16S rDNA高通量测序技术分析PIK3CA突变型(n=4)和PIK3CA野生型(n=15)之间肿瘤组织中微生物多样性和组成差异。结果:在门水平上,结直肠癌患者的肠道菌群主要由变形菌门、栖热菌门、放线菌门、厚壁菌门和拟杆菌门组成,占总群落的98%以上。Alpha多样性分析显示,PIK3CA突变型与野生型结直肠癌患者之间微生物多样性有显著性差异,且PIK3CA突变型患者中微生物多样性明显高于野生型。Beta多样性分析显示PIK3CA突变型与野生型结直肠癌患者的肿瘤微生物特征有显著性差异。Spearman关联网络分析结果显示,在PIK3CA野生型结直肠癌肿瘤组织中,双歧杆菌属与假单胞菌呈正相关。结论:PIK3CA突变型与野生型结直肠癌患者肿瘤组织中的微生物群落结构有显著性差异,且PIK3CA突变患者中的肠道微生物多样性更丰富。Objective:To study the differences in microbial characteristics between PIK3CA-mutant and-wild-type colorectal cancer.Methods:In total,19 patients with colorectal cancer who met the inclusion criteria were evaluated between December 2016 and December 2017 at Union Hospital,Tongji Medical College,Huazhong University of Science and Technology.The diversity and composition of the tumor microbiota in patients with PIK3CA-mutant(n=4)and-wild-type(n=15)colorectal cancer were explored using second-generation bacterial 16S rDNA high-throughput sequencing.Results:At the phylum level,the gut microbiota mainly comprised Proteobacteria,Thermus,Actinomycetes,Firmicutes,and Bacteroides(accounting for more than 98%of the microbiota).Alpha diversity analysis revealed significant differences in microbial diversity between PIK3CA-mutant and PIK3CA wild-type colorectal cancer patients.Further,microbial diversity in PIK3CAmutant colorectal cancer patients was significantly higher than that in wild-type patients.Beta diversity analysis revealed significant differences in tumor microbiome characteristics between PIK3CA-mutant and-wild-type colorectal cancer patients.Spearman association network analysis revealed a positive correlation between the abundance of Bifidobacterium and Pseudomonas in tissues from patients with PIK3CA wild-type colorectal cancer.Conclusions:Significant differences were observed in the microbial community structure between patients with PIK3CA-mutant and-wild-type colorectal cancer.Further,patients with PIK3CA-mutant colorectal cancer were found to exhibit higher intestinal microbial diversity.

关 键 词：高通量测序 肠道菌群 PIK3CA 结直肠癌 

分 类 号：R735.34[医药卫生—肿瘤]