功能矫形对安氏Ⅱ类错牙合模型大鼠髁突软骨生长发育及VEGF表达的影响  

Effects of Functional Orthopedics on the Growth and Development of Condylar Cartilage and the Expression of VEGF in Angel’s ClassⅡMalocclusion Model Rats

作　　者：郑新宇 ZHENG Xinyu(Department of Stomatology,Heze Hospital of Traditional Chinese Medicine,Shandong Province,Heze 274000,Shandong,China)

机构地区：[1]山东省菏泽市中医医院口腔科,山东菏泽274000

出　　处：《中国美容医学》2022年第3期103-106,共4页Chinese Journal of Aesthetic Medicine

摘　　要：目的:探讨安氏Ⅱ类错牙合模型大鼠在接受功能矫形后其髁突软骨生长发育及VEGF表达的情况。方法:安氏Ⅱ类错牙合模型大鼠30只,随机分为实验组(n=15,佩戴咬合前导矫治器,连续佩戴30 d),对照组(n=15,不佩戴咬合前导矫治器)。处死大鼠,观察两组大鼠双侧颞下颌关节髁突软骨前、中、后部生长发育情况、软组织形态、VEGF阳性表达、VEGF蛋白表达。结果:建模前两组髁突软骨厚度比较,差异无统计学意义(P>0.05),建模后实验组各部厚度明显较对照组更厚,差异具有统计学意义(均P<0.05)。实验组软骨增殖层细胞数量较对照组有所上升,细胞核有明显变大,成熟层细胞和细胞间质出现增长,肥大层细胞数量、分泌量与对照组相比呈增长趋势,软骨中部、后部纤维层变化不明显。VEGF在细胞浆和细胞膜中表达为棕黄色,两组后部阳性细胞数均高于中部,中部阳性细胞数均高于前部,其中,对照组髁突软骨前部、中部、后部VEGF蛋白阳性数均低于实验组。两组髁突软骨VEGF蛋白表达在前、中、后部呈阶梯状增长,各部对比差异均有统计学意义(均P<0.05)。结论:功能矫形可促进安氏Ⅱ类错牙合大鼠下颌髁突软骨局部生长,提高VEGF表达。Objective To investigate the growth and development of condylar cartilage and the expression of VEGF in Angel’s ClassⅡmalocclusion model rats after functional orthopedics.Methods Thirty Angle ClassⅡmalocclusion model rats were randomly divided into experimental group(with occlusal guide appliance for 30 days)and control group(without occlusal guide appliance).The rats were sacrificed,and the growth and development of the anterior,middle and posterior parts of the bilateral temporomandibular joint condylar cartilage,soft tissue morphology,VEGF positive expression and VEGF protein expression were observed in the two groups of rats.Results Before modeling,there was no significant difference in the thickness of condylar cartilage between the two groups(P>0.05).After modeling,the thickness of each part in the experimental group was significantly thicker than that in the control group(all P<0.05).Compared with the control group,the number of cells in the proliferative layer of the cartilage in the experimental group increased,the nuclei became larger,the mature layer cells and intercellular substance increased,and the number and secretion of the hypertrophic layer cells increased compared with the control group.The fibrous layer did not change significantly.The expression of VEGF in cytoplasm and cell membrane was brownish yellow.The number of positive cells in the rear of the two groups was higher than that in the middle,and the number of positive cells in the middle was higher than that in the front.Among them,the positive number of VEGF protein in the front,middle and rear of condylar cartilage in the control group was lower than that in the experimental group.The expression of VEGF protein in the anterior,middle and posterior parts of the condylar cartilage in the two groups increased in a step-like manner,and the differences in each part were statistically significant(all P<0.05).Conclusion Functional orthopedics can promote the local growth of mandibular condyle cartilage and increase the expression of VEGF

关 键 词：血管内皮生长因子 安氏Ⅱ类错牙合 功能矫形 髁突软骨 SD大鼠 

分 类 号：R783.5[医药卫生—口腔医学]