The size of cell-free mitochondrial DNA in blood is inversely correlated with tumor burden in cancer patients  

作　　者：Qin An Youjin Hu Qingjiao Li Xufeng Chen Jiaoti Huang Matteo Pellegrini Xianghong Jasmine Zhou Matthew Rettig Guoping Fan 

机构地区：[1]Department of Human Genetics,David Geffen School of Medicine,University of California Los Angeles,Los Angeles,CA 90095,USA [2]Department of Pathology and Laboratory Medicine,David Geffen School of Medicine,University of California Los Angeles,Los Angeles,CA 90095,USA [3]Department of Pathology,Duke University School of Medicine,Durham,NC 27710,USA [4]Department of Molecular,Cell,and Developmental Biology,University of California Los Angeles,Los Angeles,CA 90095-7239,USA [5]and 5Department of Urology,David Geffen School of Medicine,University of California Los Angeles,Los Angeles,CA 90095,USA

出　　处：《Precision Clinical Medicine》2019年第3期131-139,共9页精准临床医学（英文）

基　　金：We would like to thank Dr.Anthony E.Sisk Jr.at UCLA Genitourinary Pathology Unit for histological analysis.This study is funded by National Institutes of Health(Grant No:RO1 DE DE025474,P50CA092131,and P50CA211015),and CIRM Stem Cell Genomics Centers of Excellence Award.

摘　　要：Circulating cell-free DNAs(cfDNAs)are fragmented DNA molecules released into the blood by cells.Previous studies have suggested that mitochondria-originated cfDNA fragments(mt-cfDNAs)in cancer patients are more fragmented than those from healthy controls.However,it is still unknown where these short mtcfDNAs originate,and whether the length of mt-cfDNAs can be correlated with tumor burden and cancer progression.In this study,we first performed whole-genome sequencing analysis(WGS)of cfDNAs from a human tumor cell line-xenotransplantation mouse model and found that mt-cfDNAs released from transplanted tumor cells were shorter than the mouse counterpart.We next analyzed blood cfDNA samples from hepatocellular carcinoma and prostate cancer patients and found that mt-cfDNA lengths were inversely related to tumor size as well as the concentration of circulating tumor DNA.Our study suggested that monitoring the size of mt-cfDNAs in cancer patients would be a useful way to estimate tumor burden and cancer progression.

关 键 词：circulating cell-free DNA(cfDNA) tumor burden cancer progression liquid biopsy 

分 类 号：R73[医药卫生—肿瘤]