海假交替单胞菌(Pseudoalteromonas marina)pilZ基因缺失抑制厚壳贻贝附着变态  

Knockout of Pseudoalteromonas marina pilZ gene inhibited the settlement and metamorphosis of Mytilus coruscus

作　　者：张驰[1,2] 王劲松 杨金龙[1,2,3] 张俊波 万荣[4,5,6] 梁箫 Zhang Chi;Wang Jinsong;Yang Jinlong;Zhang Junbo;Wan Rong;Liang Xiao(International Research Center for Marine Biosciences,Ministry of Science and Technology,Shanghai Ocean University,Shanghai 201306,China;Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-Culture of Aquaculture Animals,Shanghai 201306,China;Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),Guangzhou 511458,China;College of Marine Sciences,Shanghai Ocean University,Shanghai 201306,China;National Engineering Research Center for Oceanic Fisheries,Shanghai 201306,China;Zhoushan Branch of National Engineering Research Center for Oceanic Fisheries,Zhoushan 316014,China)

机构地区：[1]上海海洋大学国家海洋生物科学国际联合研究中心,上海201306 [2]上海市水产动物良种创制与绿色养殖协同创新中心,上海201306 [3]南方海洋科学与工程广东省实验室(广州),广东广州511458 [4]上海海洋大学海洋科学学院,上海201306 [5]国家远洋渔业工程技术研究中心,上海201306 [6]国家远洋渔业工程技术研究中心舟山分中心,浙江舟山316014

出　　处：《海洋学报》2022年第4期95-103,共9页

基　　金：国家自然科学基金(41876159,41476131);国家重点研发计划(2019YFC0312104,2020YFD0900804);南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0402);上海市优秀学术带头人计划(20XD1421800)。

摘　　要：为探究海假交替单胞菌pilZ基因的缺失对生物被膜形成及突变菌生物被膜对厚壳贻贝幼虫附着变态的影响,本文通过同源重组构建pilZ基因缺失菌,分析了基因缺失菌生物被膜的细菌密度、膜厚、c-diGMP水平和胞外产物含量等特性的变化及其对厚壳贻贝幼虫附着变态的调控作用。结果表明:与野生型菌株相比,pilZ基因缺失菌形成的生物被膜膜厚增加、细菌数量增多,胞外产物中β-多糖、蛋白含量减少,抑制了厚壳贻贝幼虫的附着变态(p<0.05);而c-di-GMP水平、α-多糖和脂质含量无显著变化(p>0.05)。由此可见,海假交替单胞菌pilZ基因的缺失可调控细菌生物被膜形成和胞外产物包括β-多糖、蛋白质的含量,从而抑制厚壳贻贝幼虫的附着变态。To investigate the effect of Pseudoalteromonas marina pilZ gene knockout on the biofilm formation and its influence on the settlement and metamorphosis of Mytilus coruscus larvae,ΔpilZ was constructed by homologous recombination,and the changes in bacterial density,biofilm thickness,c-di-GMP level and extracellular polymeric substances(EPS) of ΔpilZ bacteria biofilm were analyzed,as well as the regulation of the settlement and metamorphosis of M.coruscus larvae.The results showed that compared with the wild-type strain,the biofilm formed by ΔpilZ strain significantly increased the biofilm thickness,the number of bacteria,while the contents ofβ-polysaccharides and proteins in EPS were decreased and the settlement and metamorphosis of M.coruscus larvae were inhibited(p<0.05).There was no significant difference in c-di-GMP level,α-polysaccharide and lipid contents(p>0.05).In conclusion,P.marina pilZ gene knockout can regulate the bacterial biofilm and the content of EPS including β-polysaccharides and proteins contents,then inhibit the settlement and metamorphosis of M.coruscus larvae.

关 键 词：pilZ 生物被膜 厚壳贻贝 附着变态 胞外产物 

分 类 号：S968.31[农业科学—水产养殖] Q938.8[农业科学—水产科学]