Injectable bioactive polymethyl methacrylate-hydrogel hybrid bone cement loaded with BMP-2 to improve osteogenesis for percutaneous vertebroplasty and kyphoplasty  

作　　者：Xin Sun Xin Zhang Xin Jiao Jie Ma Xingzhen Liu Han Yang Kangping Shen Yaokai Gan Jinwu Wang Haiyan Li Wenjie Jin 

机构地区：[1]Shanghai Key Laboratory of Orthopaedic Implants,Department of Orthopaedic Surgery,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200001,China [2]School of Biomedical Engineering,Shanghai Jiao Tong University,Shanghai 200030,China [3]Chemical and Environmental Engineering,School of Engineering,RMIT University,124 La Trobe St.,Melbourne,VIC 3000,Australia

出　　处：《Bio-Design and Manufacturing》2022年第2期318-332,共15页生物设计与制造（英文）

基　　金：supported by the National Key R&D Program of China(No.2018YFA0703000);the National Natural Science Foundation of China(Nos.82071564,82072412,and 81772326);the Fundamental Research Program Funding of Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine(No.JYZZ070);Project of Shanghai Science and Technology Commission(No.19XD1434200/18431903700)。

摘　　要：Poly methyl methacrylate(PMMA)bone cement is used in augmenting and stabilizing fractured vertebral bodies through percutaneous vertebroplasty(PVP)and percutaneous kyphoplasty(PKP).However,applications of PMMA bone cement are limited by the high elasticity modulus of PMMA,its low biodegradability,and its limited ability to regenerate bone.To improve PMMA bio activity and biodegradability and to modify its elasticity modulus,we mixed PMMA bone cement with oxidized hyaluronic acid and carboxymethyl chitosan in situ cross-linking hydrogel loaded with bone morphogenetic protein-2(BMP-2)to achieve novel hybrid cement.These fabric ated PMMA-hydrogel hybrid cements exhibited lower setting temperatures,a lower elasticity modulus,and better biodegradability and biocompatibility than that of pure PMMA cement,while retaining acceptable setting times,mechanical strength,and inj ectability.In addition,we detected release of BMP-2 from the PMMA-hydrogel hybrid cements,significantly enhancing in vitro osteogenesis of bone marrow mesenchymal stem cells by up-regulating the gene expression of Runx2,Coll,and OPN.Use of PMMA-hydrogel hybrid cements loaded with BMP-2 on rabbit femoral condyle bone-defect models revealed their biodegradability and enhanced bone formation.Our study demonstrated the favorable mechanical properties,biocompatibility,and biodegradability of fabricated PMMA-hydrogel hybrid cements loaded with BMP-2,as well as their ability to improve osteogenesis,making them a promising material for use in PKP and PVP.

关 键 词：Polymethyl methacrylate bone cement HYDROGEL Bone morphogenetic protein-2 OSTEOGENESIS Percutaneous vertebroplasty 

分 类 号：R318.08[医药卫生—生物医学工程] R687.3[医药卫生—基础医学]