新型光敏剂黑色二氧化钛介导的光动力学反应对前列腺癌细胞的体外杀伤作用研究  

Killing effects in vitro of a new photosensitizer black titanium dioxide-mediated photodynamics on prostate cancer cells

作　　者：宫满成[1] 倪文君 董文静[3] 李淼沅[1] 冯升幸 袁润强[1] Gong Mancheng;Ni Wenjun;Dong Wenjing;Li Miaoyuan;Feng Shengxing;Yuan Runqiang(Department of Urology,Zhongshan City People's Hospital,Zhongshan 528403,China;Department of Urology,Zhuhai People's Hospital,Zhuhai 519000,China;Department of Chemotherapy,Zhongshan City People's Hospital,Zhongshan 528403,China)

机构地区：[1]中山市人民医院泌尿外科,中山528403 [2]珠海市人民医院泌尿外科,珠海519000 [3]中山市人民医院化疗科,中山528403

出　　处：《肿瘤研究与临床》2022年第2期97-101,共5页Cancer Research and Clinic

基　　金：广东省中山市科技计划重大项目(2016B1003);广东省中山市科技计划(2019B1064、2018B1031);广东省中山市医学科研基金(2016J040)。

摘　　要：目的:探讨新型光敏剂黑色二氧化钛b-TP-700介导的光动力学反应对前列腺癌细胞株PC-3的体外杀伤作用。方法:利用固相原位热还原法成功制备b-TP-700。用31.25、62.5、125、250、500、1000μg/ml b-TP-700处理PC-3细胞12、24、48 h,采用CCK-8法检测细胞活性,荧光共聚焦显微镜观察PC-3细胞形态,判断b-TP-700对PC-3细胞的毒性。采用31.25、62.5、125、250、500μg/ml b-TP-700处理PC-3细胞后,激光(1 W/cm,808 nm)照射细胞1、3、5、7 min,采用CCK-8法检测细胞活性,荧光共聚焦显微镜检测250μg/ml b-TP-700培养的PC-3细胞经激光照射5 min后细胞活性氧的产生情况,以未经激光照射的细胞为对照组。结果:不同浓度b-TP-700处理PC-3细胞相同时间时,细胞活性差异均无统计学意义(F值分别为1.26、0.39、0.37,均P>0.05)。使用荧光显微镜观察1000μg/ml b-TP-700作用PC-3细胞24 h后,细胞未出现明显形态学改变,也未见明显的死细胞。随着激光激发时间的延长,相同浓度b-TP-700作用的PC-3细胞活性下降。在激光激发时间相同时,随着b-TP-700浓度的增加,PC-3细胞的活性下降(均P<0.05)。在激光照射下,250μg/ml b-TP-700作用的PC-3细胞中可以检测到明显绿色荧光,对照组细胞中几乎观察不到绿色荧光。结论:新型光敏剂b-TP-700具有良好的活性氧生成能力,其介导的光动力学作用体外对前列腺癌PC-3细胞具有明显的杀伤作用,有望成为前列腺癌治疗的新方法。Objective To investigate the killing effects in vitro of a new photosensitizer black titanium dioxide b-TP-700-mediated photodynamics on prostate cancer cell line PC-3 cells.Methods The b-TP-700 was successfully prepared by using solid phase in situ thermal reduction method.PC-3 cells were treated with 31.25,62.5,125,250,500,1000μg/ml b-TP-700 for 12,24,48 h.The cell activity was detected by using CCK-8 method,and the morphology of PC-3 cells was observed by using confocal fluorescence microscope,the toxicity of b-TP-700 to PC-3 cells was also judged.PC-3 cells were treated with different concentrations of b-TP-700(31.25,62.5,125,250,500μg/ml)and irradiated with laser(1 W/cm,808 nm)for 1,3,5,7 min.The cell activity was detected by using CCK-8 method.The production of reactive oxygen species in PC-3 cells cultured in 250μg/ml b-TP-700 after laser irradiation for 5 min was detected by using confocal fluorescence microscopy.The non-laser irradiation cells were selected as the control group.Results There were no statistically significant differences in cell activity of PC-3 cells treated with different concentrations of b-TP-700 for the same time(F value was 1.26,0.39 and 0.37,respectively;all P>0.05).PC-3 cells were treated with 1000μg/ml b-TP-700 for 24 h,no obvious morphological changes and no obvious dead cells were observed under fluorescence microscope.When PC-3 cells were treated with the same concentration of b-TP-700,the activity of PC-3 cells was decreased with the extension of laser excitation time.Under the same laser excitation time,the activity of PC-3 cells was decreased with the increase of b-TP-700 concentration(all P<0.05).Under laser irradiation,obvious green fluorescence could be detected in the PC-3 cells treated with 250μg/ml b-TP-700,while almost no green fluorescence could be observed in the control group.Conclusions A new photosensitizer b-TP-700 has the good reactive oxygen generation ability.b-TP-700-mediated photodynamics show obvious killing effects on prostate cancer PC-3 cells in v

关 键 词：前列腺肿瘤 光化学疗法 光敏剂 二氧化钛 

分 类 号：R737.25[医药卫生—肿瘤]