Quantitation of nucleoprotein complexes by UV absorbance and Bradford assay  

作　　者：Jiang Chen Hao Luo Mei Tao Zhongchuan Liu Ganggang Wang 

机构地区：[1]Key Laboratory of Environmental and Applied Microbiology,Chengdu Institute of Biology,Chinese Academy of Sciences,Key Laboratory of Environmental Microbiology of Sichuan Province,Chengdu 610041,China [2]College of Life Sciences,Sichuan University,Chengdu 610064,China [3]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Biophysics Reports》2021年第6期429-436,共8页生物物理学报（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(31470742,31700664,U1432102,31270783);an initial grant from the 100 Talents Program of the Chinese Academy of Sciences;the Key Laboratory of Environmental and Applied Microbiology at the Chinese Academy of Sciences。

摘　　要：Despite the importance of studying nucleoprotein complexes,no appropriate method for quantifying them is available.Here,a UV absorbance method using the formula“C_(mg/mL)=1.55A_(280)–0.76A_(260)”were applied to quantify nucleoprotein complexes.After modification using two paired A_(260)and A_(280)values,the UV-derived formula-based method could accurately quantify proteins in nucleoprotein complexes.Otherwise,by taking the target protein as a standard,the Bradford assay can accurately quantify proteins in nucleoprotein complexes without interference by nucleic acids.The above methods were successfully applied to measure the concentration of MtuP49-CTG complexes of Mycobacterium tuberculosis.In conclusion,both the Bradford assay and the UV-derived formula-based method were appropriate for quantifying proteins in nucleoprotein complexes,which may make contributions to explore the interactions between proteins and nucleic acids at the molecular level.

关 键 词：Nucleoprotein complex QUANTITATION Bradford assay UV-derived formula-based method 

分 类 号：Q513.1[生物学—生物化学]