Age-cumulative Effect of REC8 Reduction on Meiotic Chromosome Segregation Errors in Mice  

作　　者：Li-Yuan Tian Ling Zhang Cheng-Qiu Tao Xiao-Qi Lin Feng Zhang Bin Zhang 

机构地区：[1]Department of Obstetrics,Obstetrics and Gynecology Hospital of Fudan University,Shanghai 200011,China [2]Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases,Shanghai 200011,China

出　　处：《Reproductive and Developmental Medicine》2021年第4期193-198,共6页生殖与发育医学（英文版）

基　　金：Shanghai Municipal Science and Technology Major Project(2017 SHZDZX01)。

摘　　要：Objective:This study aimed to explore the relationship between cohesin subunit REC8 reduction and meiosis chromosome segregation errors in the ovary.Methods:Rec8^(+/-)mice were generated using CRIPSR/Cas9 gene editing.The association between age and REC8 expression levels in the ovary was determined by Western blotting.Chromosome segregation errors were investigated by immunofluorescence imaging of superovulated oocytes.Wild-type andRec8^(+/-)female mice at 5,8,20,36,and 40 weeks were used to evaluate ovarian reserve by ovarian clearing and immunolabeling.Results:Ovary REC8 expression levels gradually decreased with age,while chromosome segregation errors increased with age.Segregation errors were more common inRec8^(+/-)mice,suggesting an association with REC8 expression.The ovarian reserve capacity decreased significantly with age.The ovarian reserve inRec8^(+/-)mice was inferior to that of age-matched wild-type mice,indicating important roles of age and REC8 levels in the ovarian reserve.Conclusions:REC8 reduction has an age-cumulative effect on meiotic chromosome segregation errors in mouse ovaries.Rec8 haploinsufficiency poses a major challenge in generating normal and reproductive oocytes in aging mice.

关 键 词：Chromosome Segregation Errors COHESIN MEIOSIS REC8 

分 类 号：R714.5[医药卫生—妇产科学]