Optimization of adipose tissue-derived mesenchymal stromal cells transplantation for bone marrow repopulation following irradiation  

作　　者：Min-Jung Kim Won Moon Jeonghoon Heo Sangwook Lim Seung-Hyun Lee Jee-Yeong Jeong Sang Joon Lee 

机构地区：[1]Department of Biochemistry,Cancer Research Institute Kosin University College of Medicine,Seo-gu 49267,Busan,South Korea [2]Department of Internal Medicine,Kosin University College of Medicine,Seo-gu 49267,Busan,South Korea [3]Department of Molecular Biology and Immunology,Kosin University College of Medicine,Seo-gu 49267,Busan,South Korea [4]Department of Radiation Oncology,Kosin University College of Medicine,Seo-gu 49267,Busan,South Korea [5]Department of General Surgery,Kosin University College of Medicine,Seogu 49267,Busan,South Korea [6]Department of Ophthalmology,Gospel Hospital,Kosin University College of Medicine,Seo-gu 49267,Busan,South Korea

出　　处：《World Journal of Stem Cells》2022年第3期245-263,共19页世界干细胞杂志（英文版）（电子版）

基　　金：The Basic Science Research Program Through The National Research Foundation of Korea(NRF)Grant Funded By The Korean Government To Lee S.J.,No.2021R1F1A1052084.

摘　　要：BACKGROUND Bone marrow(BM)suppression is one of the most common side effects of radiotherapy and the primary cause of death following exposure to irradiation.Despite concerted efforts,there is no definitive treatment method available.Recent studies have reported using mesenchymal stromal cells(MSCs),but their therapeutic effects are contested.AIM We administered and examined the effects of various amounts of adipose-derived MSCs(ADSCs)in mice with radiation-induced BM suppression.METHODS Mice were divided into three groups:Normal control group,irradiated(RT)group,and stem cell-treated group following whole-body irradiation(WBI).Mouse ADSCs(mADSCs)were transplanted into the peritoneal cavity either once or three times at 5×10^(5) cells/200μL.The white blood cell count and the levels of,plasma cytokines,BM mRNA,and BM surface markers were compared between the three groups.Human BM-derived CD34+hematopoietic progenitor cells were co-cultured with human ADSCs(hADSCs)or incubated in the presence of hADSCs conditioned media to investigate the effect on human cells in vitro.RESULTS The survival rate of mice that received one transplant of mADSCs was higher than that of mice that received three transplants.Multiple transplantations of ADSCs delayed the repopulation of BM hematopoietic stem cells.Anti-inflammatory effects and M2 polarization by intraperitoneal ADSCs might suppress erythropoiesis and induce myelopoiesis in sub-lethally RT mice.CONCLUSION The results suggested that an optimal amount of MSCs could improve survival rates post-WBI.

关 键 词：Adipose tissue-derived stem cells Bone marrow suppression Mesenchymal stromal cells RADIATION Cell therapy 

分 类 号：R730.55[医药卫生—肿瘤]