羧基化聚酰胺—胺/无定形磷酸钙改善牙本质粘接的实验研究  

Experimental study on the improvement of dentin bonding by carboxylated polyamidoamine dendrimer/amorphous calcium phosphate

作　　者：黄婧娴 杨婧 谢红宇 严凯琦 谢方方[1] Huang Jingxian;Yang Jing;Xie Hongyu;Yan Kaiqi;Xie Fangfang(Department of Endodontics,Affiliated Stomatological Hospital of Guangxi Medical University,Guangxi Key Laboratory of Oral and Maxillofacial Restoration and Reconstruction Research,Guangxi Key Laboratory of Maxillofacial Surgery Disease Diagnosis and Treatment Research,Guangxi Clinical Medical Research Center of Craniomaxillofacial Malformation,Nanning 530021,China;Department of Stomatology,The Fourth Affiliated Hospital of Guangxi Medical University,Liuzhou 545005,China)

机构地区：[1]广西医科大学附属口腔医院牙体牙髓科,广西口腔颌面修复与重建研究重点实验室,广西颌面外科疾病诊治研究重点实验室,广西颅颌面畸形临床医学研究中心,南宁530021 [2]广西医科大学第四附属医院口腔科,柳州545005

出　　处：《广西医科大学学报》2022年第3期394-399,共6页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.81760204)。

摘　　要：目的:探讨粘接剂负载羧基化聚酰胺-胺/无定形磷酸钙(PAMAM—COOH/ACP)纳米复合体对树脂-牙本质粘接性能的影响。方法:收集新鲜拔除的无龋无白斑人前磨牙或第三磨牙80个并制成统一牙本质粘接试样,将80个试样随机分为4组(A组、B组、C组、D组)。A组采用自酸蚀粘接剂进行树脂粘接,B组、C组、D组分别采用含0.3wt%ACP、0.3wt%PAMAM—COOH和0.3wt%PAMAM—COOH/ACP的自酸蚀粘接剂进行树脂粘接。对各组粘接试样行剪切强度测试,并对测试结果行单因素方差分析。采用扫描电子显微镜(SEM)观察各组牙本质粘接界面断端,并在超景深三维显微系统(50倍)下统计断裂类型。结果:A组、B组、C组、D组剪切强度分别为(8.67±4.78)MPa、(10.11±3.53)MPa、(11.94±4.78)MPa、(12.01±2.91)MPa。C组和D组的剪切强度均高于A组(P<0.05),B组、C组、D组组间比较,差异无统计学意义(P>0.05)。SEM下观察断端,A组只可见树脂残留,B组可见少量疏松矿化产物生成,C组可见矿化产物深入牙本质内,D组形成致密的矿化产物且牙本质小管管径缩小。各组的断裂模式均以黏附破坏为主,除A组外,其余各组均发生少量内聚破坏或混合破坏。结论:PAMAM—COOH/ACP纳米复合体加入自酸蚀粘接剂中可提高其树脂-牙本质粘接强度。Objective:To investigate the effect of adhesive- loaded carboxylated polyamidoamine dendrimer amorphous calcium phosphate (PAMAM—COOH/ACP) nanocomposite on resin- dentin bonding performance Methods:Eighty freshly extracted cavity-free human premolar or third molar teeth were collected and made into uniform dentin bonding specimens,and the 80 specimens were randomly divided into four groups (group A group B,group C and group D).In group A,self- etching bonding agent was used for resin bonding,whereas group B,group C and group D used the nano- composite containing 0.3 wt% ACP,0.3 wt% PAMAM—COOH and 0.3 wt% PAMAM—COOH/ACP,respectively.PAMAM—COOH/ACP self- etching adhesive was used for resin bonding.Shear strength tests were performed on each group of bonded specimens,and one-way analysis of variance was performed on the test results.Scanning electron microscopy (SEM) was used to observe the fracture ends of the dentin bonding interface of each group,and the fracture types were recorded under the ultra-deep field 3D microscope system (50×).Results:The shear strengths of each group were (8.67 ± 4.78) MPa in group A,(10.11 ± 3.53) MPa in group B,(11.94 ± 4.78)MPa in group C,and (12.01 ± 2.91) MPa in group D.The shear strengths of groups C and D were higher than those of group A (P<0.05),while the differences were not statistically significant when comparing groups B,C,and D (P>0.05).When the fracture ends were observed under SEM,only resin residues were visible in group A,a small amount of loose mineralization products were seen in group B,mineralization products were seen deep into the dentin in group C,and dense mineralization products and reduced dentin tubule diameter were observed in group D.The fracture pattern of all groups was mainly adhesion damage.Except for group A,a small amount of cohesive damage or mixed damage occurred in all the groups.Conclusion:The incorporation of PAMAM—COOH/ACP nanocomposite into self-etching bonding agent can improve its resin-dentin bond strength.

关 键 词：羧基化聚酰胺—胺/无定形磷酸钙 仿生矿化 牙本质粘接 粘接强度 

分 类 号：R783.1[医药卫生—口腔医学]