超高效液相色谱-串联质谱法测定牛奶中氮氨菲啶残留量不确定度评定  

Evaluation of uncertainty for determining isometamidium residue in milk by UPLC-MS/MS

作　　者：张艳芳[1] 吕耀龙[1] 李照[2] 白荣国 ZHANG Yanfang;LYU Yaolong;LI Zhao;BAI Rongguo(Inner Mongolia Agricultural University,College of Vocational Technical Institute,Baotou 014109,China;Inner Mongolia Mengniu Dairy(Group)Co.Ltd.,Inner Mongolia,Hohhot 011517,China)

机构地区：[1]内蒙古农业大学职业技术学院,内蒙古包头014109 [2]内蒙古蒙牛乳业(集团)股份有限公司,内蒙古呼和浩特011517

出　　处：《食品与发酵工业》2022年第7期264-268,共5页Food and Fermentation Industries

摘　　要：利用超高效液相色谱-串联质谱法测定牛奶中氮氨菲啶残留量,并根据建立的数学模型评定该方法不确定度的各分量值。样品经乙腈溶液溶解,过C;小柱净化,超高效液相色谱-串联质谱测定,外标法定量。评定结果表明,标准曲线引入、回收率实验产生不确定度分量最大。当牛奶中氮氨菲啶含量为0.0178 mg/kg时,其扩展不确定度为0.0078 mg/kg(k=2)。The ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method was used to determine the isometamidium residues in milk,and the mathematic model based on this method was built for the evaluation of its uncertainty.The sample was firstly dissolved in acetonitrile solution,purified by C;column,and then determined by ultra performance liquid chromatography-tandem mass spectrometry.Finally,was quantified by external standard method.The evaluation results showed that the uncertainty components had been mainly impacted by the introduction of the standard curve and recovery experiment.The expanded uncertainty was 0.0078 mg/kg(k=2)when the content of phenanthridine in milk was 0.0178 mg/kg.

关 键 词：液质法 氮氨菲啶 不确定度 牛奶 

分 类 号：O657.63[理学—分析化学] TS252.7[理学—化学]