Genome-wide methylation profiling of early colorectal cancer using an Illumina Infinium Methylation EPIC BeadChip  

作　　者：Yu-Ling Wu Tao Jiang Wei Huang Xing-Yu Wu Peng-Jun Zhang Ya-Ping Tian 

机构地区：[1]Medical School of Chinese PLA,Beijing 100853,China [2]Medical Innovation Research Division,Chinese PLA General Hospital,Beijing 100853,China [3]Nankai University School of Medicine,Tianjin 300071,China [4]Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing),Interventional Therapy Department,Peking University Cancer Hospital and Institute,Beijing 100142,China

出　　处：《World Journal of Gastrointestinal Oncology》2022年第4期935-946,共12页世界胃肠肿瘤学杂志（英文版）（电子版）

基　　金：Supported by National Natural Science Foundation of China,No. 81972010;the National Key Research and Development Program of China,No. 2020YFC2002700, and No. 2020YFC2004604

摘　　要：BACKGROUND DNA methylation is a part of epigenetic modification,that is closely related to the growth and development of colorectal cancer(CRC).Specific methylated genes and methylated diagnostic models of tumors have become current research focuses.The methylation status of circulating DNA in plasma might serve as a potential biomarker for CRC.AIM To investigate genome-wide methylation pattern in early CRC using the Illumina Infinium Human Methylation 850K BeadChip.METHODS The 850K Methylation BeadChip was used to analyze the genome-wide methylation status of early CRC patients(n=5)and colorectal adenoma patients(n=5).Gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways enrichment analyses were performed on the selected differentially methylated sites to further discover candidate methylation biomarkers in plasma.RESULTS A total of 1865 methylated CpG sites with significant differences were detected,including 676 hypermethylated sites and 1189 hypomethylated sites.The distribution of these sites covered from the 1^(st) to 22^(nd) chromosomes and are mainly distributed on the gene body and gene promoter region.GO and KEGG enrichment analysis showed that the functions of these genes were related to biological regulation,molecular binding,transcription factor activity and signal transduction pathway.CONCLUSION The study demonstrated that the Illumina Infinium Human Methylation 850K BeadChip can be used to investigate genome-wide methylation status of plasma DNA in early CRC and colorectal adenoma patients.

关 键 词：Colorectal cancer DNA methylation 850K Methylation BeadChip Plasma colorectal adenoma 

分 类 号：R735.34[医药卫生—肿瘤]