功能核酸生物传感器在铅离子检测中的研究进展  

Research progress of functional nucleic acid biosensors in lead ion detection

作　　者：孙宏靖 孟令宇 孙春燕 SUN Hong-Jing;MENG Ling-Yu;SUN Chun-Yan(School of Chemistry and Life Science,Anshan Normal University,Anshan 114007,China;Northeast Pharmaceutical Group Liaoning Bio-medicine Co.,Ltd.,Benxi 117004,China;College of Food Science and Engineering,Jilin University,Changchun 130062,China)

机构地区：[1]鞍山师范学院化学与生命科学学院,鞍山114007 [2]东北制药集团辽宁生物医药有限公司,本溪117004 [3]吉林大学食品科学与工程学院,长春130062

出　　处：《食品安全质量检测学报》2022年第5期1471-1479,共9页Journal of Food Safety and Quality

摘　　要：铅离子(Pb^(2+))是一种广泛存在于环境中的有毒金属污染物,严重影响人类健康。传统的大型仪器检测Pb^(2)方法存在成本高、耗时长、操作过程复杂等弊端,限制了其广泛应用。现如今,生物传感器技术发展迅速,其在Pb^(2+)检测中的应用是当前的研究热点。功能核酸具有稳定性好、易化学合成和功能化修饰的优点,在生物传感器领域中占有重要地位。本文重点综述了基于Pb^(2+)依赖型脱氧核酶、G-四链体两种类型的功能核酸传感器在Pb^(2+)检测中的研究进展,根据信号传导类别的不同,主要分为荧光传感器、比色传感器、电化学传感器3大类,本文阐述了各种检测方法的优缺点,并对其应用前景和发展方向进行展望,以期为今后研发更灵敏的快速检测Pb^(2+)的功能核酸生物传感器提供新思路。Lead ion(Pb^(2+))is a toxic metal pollutant that widely exists in the environment,which seriously affects human health.Traditional methods for detecting Pb^(2+)by large-scale instruments have disadvantages such as high cost,time-consuming,and complicated operation process,which limits their wide application.Nowadays,with the rapid development of biosensor technology,its application in Pb^(2+)detection is the current research hotspot.Functional nucleic acid has the advantages of good stability,easy chemical synthesis and functional modification,and it plays an important role in the field of biosensors.This review summarized the research progress of 2 types of functional nucleic acid sensors for the detection of Pb^(2+),in which Pb^(2+)-dependent DNAzyme and G-quadruplex were introduced.According to the different types of signal transduction,it was mainly divided into 3 kinds of categories:Fluorescent sensor,colorimetric sensor and electrochemical sensor,this paper described the advantages and disadvantages of various detection methods,and prospected their application prospect and development direction,so as to provide new ideas for the future development of more sensitive and rapid functional nucleic acid biosensors for the the detection of Pb^(2+).

关 键 词：脱氧核酶 G-四链体 铅离子 生物传感器 

分 类 号：O652[理学—分析化学] TP212.3[理学—化学]