基于响应面法的杨树桑黄菌种液体培养基优化  

Optimization of Sanghuangporus vaninii Liquid Culture Medium Based on Response Surface Methodology

作　　者：滕成龙 刘迪[1,2] 尹钰涵 郭建峰 刘爽 张涵[1] 常悦 陆源添 TENG Cheng-long;LIU Di;YIN Yu-han;GUO Jian-feng;LIU Shuang;ZHANG Han;CHANG Yue;LU Yuan-tian(Agricultural College,Yanbian University,Yanji 133002,China;Fusion College,Yanbian University,Yanji 133002,China)

机构地区：[1]延边大学农学院,吉林延吉133002 [2]延边大学融合学院,吉林延吉133002

出　　处：《黑龙江农业科学》2022年第4期73-80,共8页Heilongjiang Agricultural Sciences

基　　金：延边大学校企合作项目(延大科合字[2020]2号);中韩国际农业技术合作项目(延大横向[2019]第19号);国家重点研发计划课题子课题(2017YFD0300104-02)。

摘　　要：杨树桑黄(Sanghuangporus vaninii)作为大型药用真菌,由于其生长年限长且开发不合理,导致其资源短缺,而通过液体发酵培养可以在短时间内得到大量菌丝体。本试验以菌丝体生物量为主要指标,对杨树桑黄液体培养基进行优化。通过响应面分析法对杨树桑黄液体培养基的碳源、氮源、无机盐进行筛选。结果表明:杨树桑黄ST菌株培养基的最佳碳源为葡萄糖,最佳无机盐为MgSO_(4)·7H_(2)O;最适培养基配方为葡萄糖34.44 g·L^(-1),蛋白胨7.26 g·L^(-1),酵母膏8.303 g·L^(-1),MgSO_(4)·7H_(2)O 2.618 g·L^(-1),pH自然;此时杨树桑黄生物量产量最高为2.73305 g,与预测值基本一致,优化的液体培养基可以显著提高杨树桑黄ST菌丝生物量。Sanghuangporus vaninii,a large-scale medicinal fungus,has a shortage of resources due to its long growth period and unreasonable development,and a large amount of mycelium can be obtained in a short time through liquid fermentation culture.In this experiment,the mycelial biomass was used as the main index to optimize the liquid medium of Sanghuangporus vaninii.Screening of carbon source,nitrogen source and inorganic salt in liquid medium of Sanghuangporus vaninii by response surface methodology.The results showed that the best carbon source of poplar mulberry was glucose.The best inorganic salt was MgSO_(4)·7H_(2)O,the most suitable medium formula was glucose 34.44 g·L^(-1),peptone 7.26 g·L^(-1),yeast extract 8.303 g·L^(-1),MgSO_(4)·7H_(2)O 2.618 g·L^(-1) and natural pH,at this condition,the biomass yield of Sanghuangporus vaninii was the highest at 2.73305 g,which was basically consistent with the predicted value.It is concluded that the optimized liquid medium can significantly improve Sanghuangporus vaninii mycelial biomass.

关 键 词：杨树桑黄 液体培养基 响应面设计 菌丝体生物量 

分 类 号：S76[农业科学—森林保护学]