兔膝骨性关节炎模型肠道菌群的16S rRNA检测分析  

16S rRNA detection and analysis of gut microbiota in osteoarthritis model in rabbits

作　　者：梅其杰[1] 徐文飞 延伟伟 袁长深[1] 郭锦荣[1] 黄西龙 曾超[1] 彭俊青 MEI Qi-jie;XU Wen-fei;YAN Wei-wei;YUAN Chang-shen;GUO Jin-rong;HUANG Xi-long;ZENG Chao;PENG Jun-qing(The First Affiliated Hospital,Guangxi Uni-versity of Traditional Chinese Medicine,Nanning 530023,China;Guangxi University of Traditional Chinese Medicine,Nanning 530200,China;Liyuan Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430077,China)

机构地区：[1]广西中医药大学第一附属医院,广西南宁530023 [2]广西中医药大学,广西南宁530200 [3]华中科技大学同济医学院附属梨园医院,湖北武汉430077

出　　处：《中国矫形外科杂志》2022年第6期528-532,共5页Orthopedic Journal of China

基　　金：国家自然科学基金地区基金项目(编号:82060875,82160912);广西高校中青年教师科研基础能力提升项目(编号:2021KY0298)。

摘　　要：[目的]探讨肠道菌群在兔膝骨关节炎(knee osteoarthritis,KOA)与正常对照之间的差异。[方法]20只大耳白兔,随机分成2组,12只关节内注射2%木瓜蛋白酶溶液制备KOA模型,另外8只关节内注射等量生理盐水为正常对照。采集动物粪便样本,利用16S rRNA基因靶向测序技术进行检测,分析各自粪便中肠道菌群的多样性及分布情况。[结果]KOA组粪便含有20188种物种(ASV/OTU),而对照组含有14029种(ASV/OTU)。KOA组在粪便菌群的门、属水平上显著丰富于照组(P<0.05),但两组在菌群纲、目、科、种水平上的差异无统计学意义(P>0.05)。KOA组在门水平以厚壁菌、拟杆菌的数量最多,在属水平上以瘤胃球菌属及颤螺菌属数量最多;其中明显的标志物种为变形杆菌。菌群主要与代谢通路密切相关,其中在碳水化合物代谢、氨基酸代谢及辅助因子和维生素代谢上极为突出。[结论]KOA组与对照组的肠道菌群之间存在一定差异,标志物种为变形杆菌。[Objective]To investigate the difference of gut microbiota between knee osteoarthritis(KOA)and normal control in rabbits.[Methods]A total of 20 Japanese white rabbits were randomly divided into 2 groups,of them,12 animals were injected 2%papain solution intraarticularly to prepare KOA model,while the other 8 rabbits were injected normal saline intraarticularly as normal control.Fecal sam⁃ples were collected and detected by 16S r RNA gene targeted sequencing technology to analyze the diversity and distribution of gut microbio⁃ta in their feces.[Results]The KOA group had a total of 20188 species(ASV/OTU),while the control group was of 14029 species(ASV/OTU).The fecal flora in KOA group was significantly more abundant than that in the control group in levels of phylum and genus(P<0.05),but there were no statistical significant differences in the levels of class,order,family and specie between the two groups(P>0.05).The KOA group had the greatest number of firmicutes and bacteroides at phylum level,and ruminococcus and spironella flutter at genus lev⁃el,with an obvious marker species of proteus.The gut microbiota was closely related to metabolic pathways,especially carbohydrate metab⁃olism,amino acid metabolism,cofactor and vitamin metabolism.[Conclusion]There are some differences in gut microbiota between KOA group and control group,and proteus is the marker species.

关 键 词：16S RRNA 肠道菌群 变形杆菌 膝骨关节炎 

分 类 号：R684.3[医药卫生—骨科学]