复合造模法制备SD大鼠脂肪肝模型  

SD rat fatty liver model established by compound modeling method

作　　者：钟继昌 谢金涛[2] ZHONG Jichang;XIE Jintao(North Anhui Health Vocational College,Suzhou 234000;First People’s Hospital of Suzhou City,Suzhou 234000,China)

机构地区：[1]皖北卫生职业学院 [2]宿州市第一人民医院,宿州234000

出　　处：《济宁医学院学报》2022年第2期115-118,共4页Journal of Jining Medical University

基　　金：安徽省高校自然科学研究重点项目(KJ2019 A1270)。

摘　　要：目的探讨采用脂肪乳剂、30%白酒灌胃和皮下注射CCl_(4)相结合建立SD大鼠脂肪肝动物模型的方法。方法SD大鼠随机分为正常对照组与模型组,正常对照组普通饲料喂养,模型组喂养普通饲料并给予脂肪乳剂、30%白酒灌胃,皮下注射CCl_(4),连续4周。动态观察一般情况及体重变化,HE染色观察肝脏病理动态变化过程,4周末计算肝指数,检测各组大鼠生化血清指标及肝组织中TC、TG、FFA含量。结果模型组大鼠体重低于正常对照组(P<0.05)、肝指数高于正常对照组(P<0.05);镜下观察模型组大鼠肝脏脂变程度已达1/3,符合FL的特征;与正常对照组相比,模型组大鼠血清ALT、AST活性升高(P<0.001);LDL、TC、TG含量升高(P<0.001),HDL降低(P<0.001),FFA升高(P<0.001);模型组大鼠肝组织TC、TG、FFA含量高于正常对照组(P<0.001)。结论通过4周脂肪乳剂、30%白酒灌胃与皮下注射CCl_(4)相结合的复合造模方法可成功制备SD大鼠脂肪肝动物模型。Objective To establish fatty liver(FL)model in SD rats by fat emulsion,intragastric administration of 30%alcohol and subcutaneous injection of CCl_(4).Methods SD rats were randomly divided into normal control group and model group.The normal control group was fed with ordinary fodder,while the model group was fed with ordinary fodder,together with fat emulsion and 30%alcohol,and subcutaneous injection of CCl_(4) for 4 weeks.The general situation and weight changes were dynamically observed.The dynamic changes of liver pathology were observed by HE staining.At the end of 4 weeks,the liver index was calculated,the biochemical serum indexes and the contents of TC,TG,FFA in liver tissue were measured.Results The body weight of the model group was significantly lower than that of the normal control group(P<0.05),the liver index was significantly higher than that of the normal control group(P<0.05).Microscopically,the degree of hepatic steatosis in the model group has reached 1/3,which is consistent with the characteristics of FL.Compared with the normal control group,the activities of serum ALT and AST in the model group were significantly higher(P<0.001);the contents of LDL,TC and TG increased significantly(P<0.001),HDL decreased significantly(P<0.001),FFA increased significantly(P<0.05).The contents of TC,TG and FFA in liver tissue of model group were significantly higher than those of normal control group(P<0.001).Conclusion The animal model of fatty liver in SD rats can be successfully established by the combination of fat emulsion,30%alcohol gavage and subcutaneous injection of CCl_(4) for 4 weeks.

关 键 词：脂肪肝 大鼠 动物模型 脂肪乳剂 白酒 CCl_(4) 

分 类 号：R587.1[医药卫生—内分泌]