染料木素磺酸钠对慢性脑缺血大鼠皮层神经元凋亡及Notch1信号的影响  

Effect of genisteinsodium sulfonate on apoptosis and Notch1 signal of cortical neurons in rats with chronic cerebral ischemia

作　　者：王恺迪 郭荣旺 刘锦红 谭雅薇 张丽梅 黄志华[2] 帅萍 黎晓[2] WANG Kai-di;GUO Rong-wang;LIU Jin-hong;TAN Ya-wei;ZHANG Li-mei;HUANG Zhi-hua;SHUAI Ping;LI Xiao(Gannan Medical University,Ganzhou,Jiangxi 341000;School of Basic Medicine,Gannan Medical University,Ganzhou,Jiangxi 341000;Department of Pathology,The First Affiliated Hospital of Gannan Medical University,Ganzhou,Jiangxi 341000)

机构地区：[1]赣南医学院,江西赣州341000 [2]赣南医学院基础医学院,江西赣州341000 [3]赣南医学院第一附属医院病理科,江西赣州341000

出　　处：《赣南医学院学报》2022年第2期148-153,共6页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：国家自然科学基金项目(31760290;82160688);赣州市指导性科技计划项目(GZ2021ZSF018);赣南医学院研究生创新项目(YC2020-S620);大学生创新创业训练项目(201910413009);心脑血管疾病防治教育部重点实验室项目(XN201923)。

摘　　要：目的:观察染料木素磺酸钠(Genisteinsodium sulfonate,GSS)是否可通过调节Notch1信号减少慢性脑缺血大鼠皮层神经元的凋亡。方法:采用改良型双侧颈总动脉结扎法(2-VO)制备慢性脑缺血大鼠模型,运用尼氏染色方法观察大鼠皮层神经元损伤情况,并以Real time-PCR和Western Blot方法检测大鼠皮层组织中Caspase-3、Bcl-2、Bax等凋亡相关分子mRNA和/蛋白表达,同时检测Notch1的蛋白表达。结果:经GSS治疗后,慢性脑缺血大鼠皮层神经元尼氏小体数量明显增多,并且下调了大鼠大脑皮层组织中的促凋亡分子Caspase-3和Bax的mRNA及蛋白表达水平,上调抗凋亡分子Bcl-2的mRNA及蛋白表达水平,GSS还可有效激活2-VO大鼠皮层组织中Notch1的蛋白表达。结论:GSS可能通过激活Notch1信号减轻慢性脑缺血所诱导的神经元凋亡性损伤。Objective:To observe whether genisteinsodium sulfonate(GSS)can regulate Notch1 signal to reduce the apoptosis of cortical neurons in chronic cerebral ischemia rats.Methods:The modified bilateral common carotid artery ligation(2-VO)method was used to prepare the rat model of chronic cerebral ischemia.Nissner’s staining method was used to observe the injury of cortical neurons in rats.The mRNA and protein expression levels of Caspase-3,Bcl-2,Bax were detected by Real time-PCR and Western Blot,and the activity of Notch1 was also detected by Western Blot.Results:After GSS treatment,Nissner’s bodies of cortical neurons in chronic cerebral ischemia rats were significantly increased.In addition,GSS down-regulated the mRNA and protein expression levels of pro-apoptotic molecules Caspase-3and Bax and up-regulated the mRNA and protein expression of the anti-apoptotic molecules Bcl-2 in cerebral cortex tissue of rats.GSS could also effectively activate the protein expression level of Notch1 in the cortical tissues of 2-VO rats.Conclusion:GSS may reduce neuron apoptosis induced by chronic cerebral ischemia by activating the Notch1 signal.

关 键 词：慢性脑缺血 染料木素磺酸钠 凋亡 NOTCH1 

分 类 号：R338[医药卫生—人体生理学]