产神经酸微藻Mychonastes afer的兼养固碳培养  

Mixotrophic and carbon fixation culture of nervonic acid-producing microalgae Mychonastes afer

作　　者：师晓艺 丁晓婷 万子璇 英瑜 李福利[2] 高昕[1] 范勇[2] SHI Xiaoyi;DING Xiaoting;WAN Zixuan;YING Yu;LI Fuli;GAO Xin;FAN Yong(College of Food Science and Technology,Ocean University of China,Qingdao 266071,China;Qingdao Institute of Bioenergy and Bioprocss Technology,Chinese Academy of Science,Qingdao 266101,China;Qingdao Institute for Food and Drug Control,Qingdao 266071,China)

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266071 [2]中国科学院青岛生物能源与过程研究所,山东青岛266101 [3]青岛市食品药品检验研究院,山东青岛266071

出　　处：《南方水产科学》2022年第2期134-141,共8页South China Fisheries Science

基　　金：国家重点研发计划项目(2019YFD0901904);国际科技创新合作重点专项(2018YFE 0107200)。

摘　　要：微藻是水体中重要的生产者和环境调节因素,作为饵料和调水剂在水产养殖中起着重要作用。微藻的培养方式多样,探究了微藻Mychonastes afer和小球藻Chlorella sorokiniana在不同营养方式及通气情况的生长与光合能力。在自养、兼养和异养,以及不同浓度二氧化碳(CO;)通气的条件下,分别测定了两株藻的生长曲线、光合电子传递效率、有机碳源利用率、光合放氧和呼吸耗氧速率,并着重对M.afer的油脂组分进行分析。结果表明小球藻C.sorokiniana可以摆脱对光能的依赖,完全依靠有机碳源营异养生长,而在此条件下M.afer几乎不能生长。有机碳源的加入,对两株藻的光合系统活性均产生了一定程度的抑制作用,使光合电子传递效率以及光合放氧水平下降;同时发现在兼养条件下,高浓度CO;最有利于M.afer生长,促进了细胞对葡萄糖的利用效率,且油脂和神经酸产量提高。研究表明两株藻在协同利用外源有机碳源和自身光合系统方面存在显著差异,并探究出一种用于生产天然神经酸的M.afer最佳兼养条件。Microalgae are a group of single cell microorganism,which play an important role in aquatic production such as aquatic feeding and water regulating.This research focuses on the growth and photosynthetic capacity of Mychonastes afer and Chlorella sorokiniana under different nutrition and aeration conditions.The microalgae were cultivated under autotrophic,mixotrophic or heterotrophic conditions,respectively.And the influence of CO;concentration was also investigated.The growth curve,photosynthetic electron transfer rate,organic carbon source utilization,photosynthetic oxygen evolution and respiratory oxygen consumption rate were measured to inflect the differences between M.afer and C.sorokiniana,and the emphasis was given to lipid components of M.afer.The results show that C.sorokiniana could grow under heterotrophic condition without light,while M.afer could not.The photosynthetic system of the two species were both inhibited by organic carbon sources,displaying lower efficiency of photosynthetic electron transfer rates and slower photosynthetic oxygen evolution rates.Besides,it is found that under mixotrophy condition,high concentration of CO;was beneficial to the growth of M.afer,which promoted cell utilization of glucose,and increased the production of lipid and nervonic acid.The study explores a mixotrophic condition for M.afer to produce natural nervonic acid,shows significant differences between the two algae species in photosynthetic system,and explores the cooperative utilization of external organic carbon sources.

关 键 词：微藻Mychonastes afer 兼养培养 二氧化碳 呼吸作用 光合作用 

分 类 号：S917.3[农业科学—水产科学]