不同贮藏条件对蒙古黄芪种子生理生化及活力的影响  

Effects of Different Storage Conditions on Physiology and Biochemistry and Seed Vigor of Astragalus mongolicus

作　　者：鲁海坤 姜业成 隋昕 于芸泽 于营[1] 郭靖[1] LU Haikun;JIANG Yecheng;SUI Xin;YU Yunze;YU Ying;GUO Jing(Institute of Special Animal and Plant Sciences of CAAS,Changchun Jilin 130000,China;Institute of Agricultural Resources and Environment,Jilin Academy of Agricultural Sciences,Changchun 130000,China)

机构地区：[1]中国农业科学院特产研究所,吉林长春130000 [2]吉林省农科院资源与环境研究所,长春130000

出　　处：《种子》2022年第3期18-24,共7页Seed

基　　金：吉林省科技发展计划项目(20180201034 YY);中国农业科学院科技创新工程(CAAS-ASTIP-2016-ISAPS)。

摘　　要：为探究蒙古黄芪种子贮藏过程中活力和生理生化指标的变化,揭示其老化机理、筛选适宜贮藏的含水量和温度,以不同含水量(3.7%,6.6%,9.8%)的蒙古黄芪种子为试材,于不同条件(室内、短期库、中期库、长期库)下贮藏12个月,测定种子发芽率和生理生化指标。结果表明,不同贮藏条件和不同含水量的种子发芽率均不断降低,在这4种条件下贮藏12个月后,含水量为9.8%的种子发芽率分别为27.7%、39.3%、42.3%、47.3%;含水量为6.6%的种子发芽率分别为32.3%、42.3%、44.0%、53.3%;含水量为3.7%的种子发芽率分别为35%、44.7%、49.7%、62.0%;贮藏过程中种子活性氧含量升高,SOD活性升高,APX活性不断下降,有毒物质MDA逐渐积累,可溶性蛋白和可溶性糖含量下降;种子含水量和贮藏温度协同影响种子活力,低含水量且低温条件更有利于保持种子活力,大幅减小种子贮藏过程中发芽率的下降幅度。因此,蒙古黄芪种子适宜贮藏条件为:含水量3.7%、温度-18℃。To explore the vigor and physiological and biochemical changes of Astragalus mongolicus seed during storage process,to reveal the mechanism of aging and screen suitable water content and temperature for storage,the seeds of A.mongholicus with different water content(3.7%,6.6%and 9.8%)were stored for 12 months in different conditions(indoor,short-term storage,medium-term storage and long-term storage),and the germination rate and physiological and biochemical indexes of the seeds were determined.The results showed that the germination rate of seeds under different storage conditions and different water content were decreased continuously.After 12 months storage under the four conditions,the germination rate of seeds with 9.8%water content were 27.7%,39.3%,42.3%and 47.3%,respectively.The germination rate of seeds with 6.6%water content were 32.3%,42.3%,44.0%and 53.3%,respectively.The germination rates of seeds with 3.7%water content were 35%,44.7%,49.7%and 62.0%,respectively.During the storage process,the content of reactive oxygen increased and the activity of SOD increased while the activity of APX decreased.In addition,the toxic substance MDA accumulated gradually,and the content of soluble protein and soluble sugar decreased.Seed water content and storage temperature had a synergistic effect on seed vigor.Low water content and low temperature were more conducive to maintaining seed vigor,which greatly reduced the decrease of seed germination rate during seed storage.Therefore,the suitable storage conditions of A.mongolicus seeds were 3.7%water content and-18℃temperature.

关 键 词：蒙古黄芪 贮藏条件 种子老化 生理生化 种子活力 

分 类 号：S567.239[农业科学—中草药栽培]