粗糙脉孢菌驱动蛋白-1在菌丝极性生长中的功能  

Functional analysis of kinesin-1 in hyphal polarized growth of Neurospora crassa

作　　者：孙海涛[1] 陈志玲[1] SUN Haitao;CHEN Zhiling(College of Life Sciences,Capital Normal University,Beijing 100048)

机构地区：[1]首都师范大学生命科学学院,北京100048

出　　处：《首都师范大学学报（自然科学版）》2022年第2期49-55,共7页Journal of Capital Normal University：Natural Science Edition

基　　金：2020首都师范大学实验室开放基金项目。

摘　　要：为了研究驱动蛋白-1的基因(Kin-1)在菌丝极性生长中的功能,本文以粗糙脉孢菌(Neurospora crassa)菌株为材料,利用同源重组技术尝试获得其敲除突变菌株Kin-1^(KO),并分析菌株87-3、Ku70^(RIP)和Kin-1^(KO)的菌丝生长情况.结果表明,与菌株87-3和Ku70^(RIP)相比,菌株Kin-1^(KO)的菌落生长缓慢、质地致密,菌丝生长速率下降,菌丝直径变粗、分枝增多.本研究成果获得了粗糙脉孢菌Kin-1敲除突变株Kin-1^(KO),证实Kin-1对于粗糙脉孢菌菌丝的生长至关重要.To investigate the function of kinesin-1(Kin-1)in the polar growth of mycelia,Neurospora crassa(N.crassa)strain was used as material to obtain its knockout mutant Kin-1^(KO)by homologous recombination technology,and the mycelial growth of strain 87-3,Ku70^(RIP) and Kin-1^(KO)was analyzed.The results showed that compared with strain 87-3 and Ku70^(RIP),the colony of strain Kin-1^(KO)grew slowly and compactly,the growth rate of mycelia decreased,the diameter of hyphae became thicker and the branches increased.In this study,the knock-out mutant Kin-1^(KO)of Kin-1 in Neurospora crassa was obtained,which confirmed that Kin-1 was very important for the growth of Neurospora crassa.

关 键 词：驱动蛋白-1 极性生长 粗糙脉孢菌 

分 类 号：Q71[生物学—分子生物学]