微核检测在辣条食用安全性评估中的应用  

Micronucleus test technique in the food safety testing of spicy strip

作　　者：赵昕[1] 陈宣齐 张燕翔[3] 李雅轩[1] ZHAO Xin;CHEN Xuanqi;ZHANG Yanxiang;LI Yaxuan(College of Life Sciences,Capital Normal University,Beijing 100048;Beijing NO.10 Middle School,Beijing 100072;Elementary Education College,Capital Normal University,Beijing 100048)

机构地区：[1]首都师范大学生命科学学院,北京100048 [2]北京市第十中学,北京100072 [3]首都师范大学初等教育学院,北京100048

出　　处：《首都师范大学学报（自然科学版）》2022年第2期56-60,共5页Journal of Capital Normal University：Natural Science Edition

基　　金：2021首都师范大学教学改革项目。

摘　　要：为了研究辣条的食品安全性,利用微核检测技术,分析不同稀释倍数的辣条提取液对蚕豆根尖的遗传毒理作用.实验分为:空白组、对照组和实验1~5组(按稀释倍数由高到低排序),分别给予等体积的H_(2)O、5 mg/L环磷酰胺和稀释200~0倍的辣条提取液处理蚕豆根尖,并取根尖细胞进行观察,对不同组数据进行统计学分析.结果表明:与空白组相比,实验1~5组均可诱导微核产生,具有一定遗传毒性;实验2、3和4组的微核数显著增加(t=1.96、2.49和3.42,P<0.05),实验1和5组的微核数增加但结果差异无统计学意义(P>0.05).与对照组相比,实验1和2组遗传毒性具有显著差异(t=−2.34和−2.07,P<0.05),实验3、4和5组遗传毒性与对照组相当且差异无统计学意义(P>0.05).在实验浓度范围内,辣条提取液诱导产生的微核数随溶液稀释倍数的降低而增加,并呈现剂量效应.In order to study the food safety of spicy strip,the micronucleus detection technology was used to test the genotoxicity of different concentrations of spicy strip extraction.In this study we set the blank group,positive control group and experimental group 1 to 5(ordered by dilution ratio from high to low).Root tips of Vicia faba were treated with deionized water,5 mg/L cyclophosphamide and different dilution ratio(200 to 0 times)spicy strip extraction,then root-tip cells were observed under the microscope.The result showed that compared with the blank group,the extracts of experimental group 1 to 5 could induce micronucleus generation and had certain genetic toxicity.The number of micronuclei of experimental group 2,3 and 4 were significantly increased(t=1.96,2.49,3.42,P<0.05).The number of micronuclei of experimental group 1 and 5 were increased,but the difference was not significant(P>0.05).Compared with the positive control group,there were significant difference in experimental group 1 and 2(t=−2.34,−2.07,P<0.05),while the experimental group 3,4 and 5 had the similar genotoxicity with the positive control group and no statistical significance was observed(P>0.05).In the range of experimental concentration,the number of micronuclei increased with the decrease of dilution ratio of spicy strip extraction,and showed does effect.

关 键 词：微核 辣条 遗传毒性 安全评价 

分 类 号：X171[环境科学与工程—环境科学] X835