Chitin synthase 1 and five cuticle protein genes are involved in serosal cuticle formation during early embryogenesis to enhance eggshells in Nilaparvata lugens  

作　　者：Jia-Bao Lu Jian-Sheng Guo Xuan Chen Chen Cheng Xu-Mei Luo Xiao-Ya Zhang Bernard Moussian Jian-Ping Chen Jun-Min Li Chuan-Xi Zhang 

机构地区：[1]State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province,Institute of Plant Virology,Ningbo University,Ningbo,Zhejiang Province,China [2]Institute of Insect Science,Zhejiang University,Hangzhou,China [3]School of Medicine,Zhejiang University,Hangzhou,China [4]UniversitéCôte d'Azur,CNRS,Inserm,Institute of Biology Valrose,Nice,France

出　　处：《Insect Science》2022年第2期363-378,共16页昆虫科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(31630057 and 31871954).

摘　　要：Many holo-and hemimetabolous insects enhance their eggshells during embryogenesis by forming a serosal cuticle(SC).To date,scholarly understanding of the SC composition and SC-related gene functions has been limited,especially for hemimetabolous insects.In this study,we initially performed transmission electron microscopic(TEM)observation and chitin staining of the SC in Nilaparvata lugens,a hemimetabolous rice pest known as the brown planthopper(BPH).We confirmed that the SC was a chitin-rich lamellar structure deposited gradually during the early embryogenesis.Parental RNA interference(RNAi)against Nilaparvata lugens chitin synthase 1(NlCHS1)in newly emerged and matured females resulted in decreases of egg hatchability by 100%and 76%,respectively.Ultrastructural analyses revealed loss of the lamellar structure of the SC in dsNlCHS1-treated eggs.According to temporal expression profiles,five cuticle protein coding genes,NlugCpr1/2/3/8/90,were specifically or highly expressed during the SC formation period,and NlugCpr1/2/3/90 were further detected in 72 h eggshells extract by ultra-performance liquid chromatography-tandem mass spectrometry/mass spectrometry.NlugCpr2/3/90 were likely three SC-specific cuticle proteins.TEM observations of the SC following parental RNAi against NlugCpr1/2/3/8/90 demonstrated that NlugCpr3/8/90 were essential for SC formation.The study provided an understanding of the SC formation process and SC-related cuticle proteins in BPHs,which offer potential targets for pest control in the egg stage as well.

关 键 词：chitin synthase 1 cuticle protein EGGSHELL EMBRYOGENESIS Nilaparvata lugens serosal cuticle 

分 类 号：Q96[生物学—昆虫学]