长链非编码RNA01139靶向调控微小RNA-300对口腔鳞癌顺铂耐药细胞增殖、迁移和侵袭的影响  

Effects of long non-coding RNA LINC01139 in regulating proliferation,migration and invasion of oral squamous cell carcinoma cisplatin-resistant cells by targeting microRNA-300

作　　者：邓琳 谢陆莉 李鲲 DENG Lin;XIE Luli;LI Kun(Department of Stomatology,the First People′s Hospital of Longquanyi District of Chengdu in Sichuan Province,Chengdu,Sichuan,610100)

机构地区：[1]四川省成都市龙泉驿区第一人民医院口腔科,四川成都610100

出　　处：《实用临床医药杂志》2022年第5期86-90,95,共6页Journal of Clinical Medicine in Practice

摘　　要：目的探讨长链非编码RNA01139(LINC01139)调控微小RNA-300(miR-300)对口腔鳞癌顺铂(DDP)耐药细胞(CAL-27/DDP)增殖、迁移和侵袭的影响。方法采用实时荧光定量聚合酶链反应(qRT-PCR)检测CAL-27/DDP及其亲本细胞株CAL-27中LINC01139和miR-300的表达水平。将CAL-27/DDP分为DDP+si-NC组、DDP+si-LINC01139组、DDP+miR-NC组、DDP+miR-300组、DDP+si-LINC01139+anti-miR-NC组、DDP+si-LINC01139+anti-miR-300组。采用噻唑兰(MTT)法检测细胞增殖抑制率;Transwell实验检测迁移和侵袭细胞数。采用双荧光素酶报告实验和qRT-PCR确定LINC01139与miR-300的靶向关系。结果与CAL-27细胞比较,CAL-27/DDP中LINC01139的表达升高,miR-300的表达降低,差异有统计学意义(P<0.05)。与DDP+si-NC组比较,DDP+si-LINC01139组CAL-27/DDP增殖抑制率升高,迁移和侵袭细胞数减少,差异有统计学意义(P<0.05)。与DDP+miR-NC组比较,DDP+miR-300组CAL-27/DDP增殖抑制率升高,迁移和侵袭细胞数减少,差异有统计学意义(P<0.05)。与DDP+si-LINC01139+anti-miR-NC组比较,DDP+si-LINC01139+anti-miR-300组CAL-27/DDP增殖抑制率降低,迁移和侵袭细胞数增加,差异有统计学意义(P<0.05)。结论抑制LINC01139可通过上调miR-300抑制CAL-27/DDP的增殖、迁移和侵袭。Objective To investigate the effect of long intergenic non-coding RNA LINC01139(LINC01139)in regulating the proliferation,migration and invasion of oral squamous cell carcinoma cisplatin(DDP)-resistant cells(CAL-27/DDP)by regulating microRNA-300(miR-300).Methods Real-time fluorescent quantitative polymerase chain reaction(qRT-PCR)was used to detect the expression levels of LINC01139 and miR-300 in CAL-27/DDP and its parental cell line CAL-27.CAL-27/DDP was divided into DDP+si-NC,DDP+si-LINC01139,DDP+miR-NC,DDP+miR-300,DDP+si-LINC01139+anti-miR-NC,DDP+si-LINC01139+anti-miR-300 groups.The methyl thiazolyl tetrazolium(MTT)method was used to detect the cell proliferation inhibition rate;Transwell assay was used to detect the number of migrating and invading cells.The dual luciferase reporting experiment and qRT-PCR were used to detect the targeting relationship between LINC01139 and miR-300.Results Compared with CAL-27 cells,the expression of LINC01139 in CAL-27/DDP was increased,while the expression of miR-300 was decreased(P<0.05).Compared with the DDP+si-NC group,the proliferation inhibition rate of CAL-27/DDP in the DDP+si-LINC01139 group was increased,and the migrating and invading cells were reduced(P<0.05).Compared with the DDP+miR-NC group,the proliferation inhibition rate of CAL-27/DDP in the DDP+miR-300 group was increased,and the number of migrating and invading cells was reduced(P<0.05).Compared with the DDP+siLINC01139+anti-miR-NC group,the proliferation inhibition rate of CAL-27/DDP in the DDP+siLINC01139+anti-miR-300 group was decreased,the migrating and invading cells were increased(P<0.05).Conclusion Inhibition of LINC01139 inhibits the proliferation,migration and invasion of oral squamous cell carcinoma cisplatin-resistant cells by up-regulating miR-300.

关 键 词：长链非编码RNA01139 微小RNA-300 口腔鳞癌顺铂耐药细胞 增殖 迁移 侵袭 

分 类 号：R739.8[医药卫生—肿瘤] R730.2[医药卫生—临床医学]