干扰素调节因子1对舌鳞癌细胞增殖侵袭和迁移的影响  

Effects of IRF1 on Proliferation,Migration and Invasion of Tongue Squamous Cell Carcinoma Cells

作　　者：柳雪[1] 刘慧[1] 霍峰[2] 徐树雷[1] 陶亚东 LIU Xue;LIU Hui;HUO Feng;XU Shu-lei;TAO Ya-dong(University Health Service,Chengde Medical University,Chengde,Hebei,067000,China;Department of dental,The affiliated hospital of Chengde Medical University)

机构地区：[1]承德医学院校医院,河北承德067000 [2]承德医学院附属医院

出　　处：《承德医学院学报》2022年第2期102-106,共5页Journal of Chengde Medical University

基　　金：河北省教育厅一般项目(QN2019079);承德医学院自然科学基金项目(201722)。

摘　　要：目的 研究干扰素调节因子对舌鳞癌细胞的侵袭和迁移的影响。方法 采用实时定量PCR和免疫组化检测IRF1在舌鳞癌组织中的表达水平,通过构建IRF1的过表达和IRF1沉默的质粒,在舌鳞癌细胞Tca8113中过表达IRF1或降低IRF1的表达水平后,检测其对Tca8113细胞增殖,侵袭和迁移的影响。结果 IRF1在舌鳞癌组织中表达异常降低,实时定量PCR和免疫组化结果均明显低于癌旁对照组。成功构建IRF1的过表达和沉默质粒,生长曲线实验显示,过表达IRF1促进Tca8113细胞的增殖,沉默IRF1抑制Tca8113细胞的增殖。划痕实验表明,过表达IRF1促进Tca8113细胞的迁移,沉默IRF1抑制Tca8113细胞的迁移。Transwell实验表明,过表达IRF1促进Tca8113细胞的侵袭,沉默IRF1抑制Tca8113细胞的侵袭。结论 在舌鳞癌的发生发展中,IRF1作为抑癌基因发挥作用。在舌鳞癌组织中,IRF1的表达水平降低。Objective To explore the influence of IRF1 on invasion and migration of tongue squamous cell carcinoma cells.Methods The expression level of IRF1 in tongue squamous cell carcinoma was detected by real-time quantitative PCR and immunohistochemistry.Construction of plasmids for IRF1 overexpression and silencing.Tca8113 was tongue squamous carcinoma cell lines.The effect of over-expressing IRF1 or reducing IRF1 expression level on Tca8113 cell proliferation,invasion and migration was detected.Results The expression of IRF1 in tongue squamous cell carcinoma tissues decreased abnormally,and the results of real-time quantitative PCR and immunohistochemistry were significantly lower than those in para-cancer tissue.The IRF1 over-expression and silencing plasmid were successfully constructed.Growth curve experiments showed that over-expression of IRF1 promoted proliferation of Tca8113 cells,while knockdown of IRF1 inhibited proliferation of Tca8113 cells.The scratch test showed that over-expression of IRF1 promoted the migration of Tca8113 cells,and knockdown IRF1 inhibited the migration of Tca8113 cells.Transwell experiments showed that over-expression of IRF1 promoted the invasion of Tca8113 cells,while knockdown of IRF1 inhibited the invasion of Tca8113 cells.Conclusion IRF1 played a role as a tumor suppressor gene in the development of tongue squamous cell carcinoma.The expression level of IRF1 decreased in tongue squamous cell carcinoma.

关 键 词：IRF1 舌鳞癌 增殖 迁移 侵袭 

分 类 号：R739.8[医药卫生—肿瘤]