子宫内膜异位症Treg细胞上调的机制研究  

The Research of Up-regulation of Treg Cells in Endometriosis

作　　者：朱义保[1] 吴斐 万磊[1] ZHU Yibao;WU Fei;WAN Lei(Jiangxi Maternal and Child Care Service Hospital,Nanchang,330006)

机构地区：[1]江西省妇幼保健院,330006

出　　处：《实用癌症杂志》2022年第4期528-531,共4页The Practical Journal of Cancer

基　　金：国家自然科学基金(编号:81660736)。

摘　　要：目的研究子宫内膜异位症Treg细胞上调的原因。方法选41例子宫内膜异位症患者,其中16例术前经过GnRH-a治疗的为治疗组,另25例术前未经治疗的为未治疗组,同时选25例子宫肌瘤患者为对照组,通过采血、腹腔液取样,用化学发光、流式细胞和ELISA方法检查化验,观察治疗组、未治疗组和对照组的基础雌二醇水平、腹腔液Treg细胞百分比及与Treg细胞诱导有关的IL-10、TGF-β、PGE-2炎症因子水平。结果经GnRH-a治疗后,治疗组的基础雌二醇下降显著,相比于治疗组治疗前、未治疗组及对照组,差异有统计学意义(P=0.00)。未治疗组Treg细胞百分比明显升高,相比于治疗组治疗后、对照组,差异有统计学意义(P<0.01);与Treg细胞诱导有关的TGF-β、PGE-2在未治疗组显著增高,相比于对照组、治疗组治疗后,差异有统计学意义(P=0.00)。IL-10在治疗组、未治疗组、对照组中的差异无统计学意义(P>0.05)。结论子宫内膜异位症经GnRH-a治疗后,Treg细胞及TGF-β、PGE-2炎症因子水平下降,提示患者腹腔存在炎症,Treg细胞的上调可能与其诱导的炎症因子的升高有关。Objective To investigate the causes of Treg elevation in endometriosis.Methods 41 cases of endometriosis were selected,16 cases of which treated with GnRH-a were the treatment group,25 cases of which not treated with GnRH-a were the non treatment group,and 25 cases of hysteromyoma were selected as the control group.Samples were collected from blood and peritoneal fluid.Chemiluminescence,flow cytometry and ELISA were used to examine these samples,the levels of estradiol,Treg cells and IL-10,TGF-beta,PGE-2 which related to Treg cells induction were then observed.Results the basic estradiol of the treatment group decreased after GnRH-a treatment compared to the non-treatment group and the control group,the difference shown statistical significance(P=0.00).Treg cells in the non-treatment group,in contrast to the control group and the treatment group increased obviously,and the difference made statistical significance too(P<0.01).Further ivestigation revealed that TGF-β,PGE-2 of the non-treatment group which associated to Treg cells induction increased,and made statistical difference compared to control group and the treatment group(P=0.00),while IL-10 varied a little between treatment group,non-treatment group and the control group,and made no statistical significance(P>0.05).Conclusion Treg cells and TGF-β,PGE-2 are decreased after GnRH-a treatment,indicated that inflammation existed in abdominal cavity of patients,and the up-regulation of Treg cells may be related to the increase of induced inflammatory factors.

关 键 词：子宫内膜异位症 TREG细胞 GNRH-A 免疫调节 

分 类 号：R737.33[医药卫生—肿瘤]