非小细胞肺癌患者CX3CL1表达与肿瘤侵袭和迁移能力相关性分析  

Correlation analysis of CX3CL1 expression and tumor invasion and migration ability in patients with non-small cell lung cancer

作　　者：尚俊依[1] 杨正波[2] SHANG Jun-yi;YANG Zheng-bo(Department of Respiratory and Critical Care Medicine,Henan Provincial People's Hospital,Zhengzhou,Henan 450003;Department of Anesthesia and Perioperative Medicine,Henan Provincial People's Hospital,Zhengzhou,Henan 450003,China)

机构地区：[1]河南省人民医院呼吸与危重症医学科,河南郑州450003 [2]河南省人民医院麻醉与围术期医学科,河南郑州450003

出　　处：《热带医学杂志》2022年第2期184-188,202,共6页Journal of Tropical Medicine

基　　金：2018年度河南省卫生计生科技英才海外研修项目(2018095)。

摘　　要：目的 探究非小细胞肺癌患者趋化因子C-X3-C配体1(CX3CL1)表达与肿瘤侵袭、迁移能力相关性及其作用机制。方法 选取河南省人民医院2018年3月-2021年3月收治的非小细胞肺癌患者100例进行研究,均进行CX3CL1表达检测,根据检测结果将所有患者分为CX3CL1高表达组(CX3CL1>0.8 mg/mL,n=50)及CX3CL1低表达组(CX3CL1≤0.8 mg/mL,n=50),比较两组临床资料,肿瘤侵袭、迁移相关指标及实验结果,并进行相关性分析。结果 CX3CL1低表达组肿瘤直径、外周血血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(BFGF)、肝癌衍生生长因子(HDGF)、单核细胞趋化蛋白-1(MCP-1)、转化生长因子-β(TGF-β)、基质金属蛋白酶抑制因子(TIMP-1)水平、微血管密度、基质金属蛋白酶-9(MMP-9)、肿瘤坏死因子受体相关蛋白1(TRAP1)m RNA、Src、局部粘着斑激酶(FAK)蛋白及细胞迁移实验中细胞平均迁移数低于CX3CL1高表达组,CX3CL1低表达组脏器转移、骨转移及临床分期Ⅲ、Ⅳ期例数低于CX3CL1高表达组,差异均有统计学意义(P<0.05)。E-cadherin、转录因子Krüppel样因子4(KLF4)m RNA显著高于CX3CL1高表达组,差异有统计学意义(P<0.05);Pearson相关性结果显示,CX3CL1表达与患者肿瘤直径、VEGF、BFGF、HDGF、MCP-1、TGF-β、TIMP-1水平,TRAP1 m RNA及划痕实验结果呈正相关,与E-cadherin、KLF4 mRNA表达呈负相关(P<0.05)。结论 非小细胞肺癌患者CX3CL1表达与其肿瘤侵袭、迁移能力具有相关性,CX3CL1可能可通过影响患者肿瘤迁移相关mRNA及肿瘤侵袭相关因子及SRC/FAK蛋白通路表达促进非小细胞肺癌患者肿瘤转移。Objective To explore the correlation between the expression of C-X3-C ligand 1(CX3CL1)and tumor invasion and migration in patients with non-small cell lung cancer and its mechanism.Methods 100 patients with non-small cell lung cancer admitted to Henan Provincial People ’ s Hospital from March 2018 to March 2021 were selected as research subjects.All patients were tested for CX3CL1 expression and all patients were divided into CX3CL1 high expression group(CX3CL1>0.8 mg/mL,n=50)and CX3CL1 low expression group(CX3CL1≤0.8 mg/mL,n=50).The clinical data of the two groups,tumor invasion and migration related indicators and experimental results were compared and their correlations were analyzed.Results The tumor diameter,the levels of vascular endothelial growth factor(VEGF),basic fibroblast growth factor(BFGF),hepatocarcinoma-derived growth factor(HDGF),monocyte chemotactic protein-1(MCP-1),transforming growth factor-β(TGF-β),tissue inhibitor of metalloproteinases-1(TIMP-1),microvessel density,matrix metallopeptidase-9(MMP-9),tumor necrosis factor re-ceptor-associated protein 1(TRAP1)mRNA,Src,focal adhesion kinase(FAK)protein and the average number of cell migration in the cell migration experiment were lower in the CX3CL1 low expression group than those in the CX3CL1 high expression group.The number of organ metastasis,bone metastasis and clinical stage Ⅲ andⅣ cases in the CX3CL1 low expression group were lower than those of the CX3CL1 high expression group,the differences were statistically significant(P<0.05).E-cadherin,and Krüppel-like factor 4(KLF4)mRNA levels were significantly higher than those in the CX3CL1 high expression group(P<0.05).Pearson correlation results showed that the expression of CX3CL1 was positively correlated with the tumor diameter,the levels of VEGF,BFGF,HDGF,MCP-1,TGF-β,TIMP-1,TRAP1 mRNA and the results of the scratch test,and negatively correlated with the expression of E-cadherin and KLF4 mRNA levels(P<0.05).Conclusions The expression of CX3CL1 in patients with non-small cell lu

关 键 词：非小细胞肺癌 CX3CL1 肿瘤侵袭 迁移 

分 类 号：R734.2[医药卫生—肿瘤]